ArticleJournal of bacteriology2012
Function and regulation of isoforms of carbon monoxide dehydrogenase/acetyl coenzyme A synthase in Methanosarcina acetivorans.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- Interface swapping orchestrates carbon transfer in the archaeal acetyl-CoA decarbonylase/synthase.bioRxiv : the preprint server for biology · 2026Article
- Article
- Genus-specific remodeling of carbon and energy metabolism facilitates acetoclastic methanogenesis inJournal of bacteriology · 2026Article
- Genus-specific remodeling of carbon and energy metabolism facilitates acetoclastic methanogenesis inbioRxiv : the preprint server for biology · 2025Article
- Mer overexpression inApplied and environmental microbiology · 2025Article
- A promoter-RBS library for fine-tuning gene expression inApplied and environmental microbiology · 2024Article
- Acetate Production from Syngas Produced from Lignocellulosic Biomass Materials along with Gaseous Fermentation of the Syngas: A Review.Microorganisms · 2023Review
- Diversity and Metabolism of Microbial Communities in a Hypersaline Lake along a Geochemical Gradient.Biology · 2022Article
- Insights into the biotechnology potential ofFrontiers in microbiology · 2022Review
- The monofunctional CO dehydrogenase CooS is essential for growth of Thermoanaerobacter kivui on carbon monoxide.Extremophiles : life under extreme conditions · 2021Article
- New Microbial Lineages Capable of Carbon Fixation and Nutrient Cycling in Deep-Sea Sediments of the Northern South China Sea.Applied and environmental microbiology · 2019Article
- Methanogenesis on Early Stages of Life: Ancient but Not Primordial.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2018Article
- Structural and Phylogenetic Diversity of Anaerobic Carbon-Monoxide Dehydrogenases.Frontiers in microbiology · 2018Article
- Short-Term Exposure of Paddy Soil Microbial Communities to Salt Stress Triggers Different Transcriptional Responses of Key Taxonomic Groups.Frontiers in microbiology · 2017Article
- Genome-Scale Metabolic Modeling of Archaea Lends Insight into Diversity of Metabolic Function.Archaea (Vancouver, B.C.) · 2017Review
- Genome-wide gene expression and RNA half-life measurements allow predictions of regulation and metabolic behavior in Methanosarcina acetivorans.BMC genomics · 2016Article
- Insights into CO2 Fixation Pathway of Clostridium autoethanogenum by Targeted Mutagenesis.mBio · 2016Article
- Metagenomics Reveals Pervasive Bacterial Populations and Reduced Community Diversity across the Alaska Tundra Ecosystem.Frontiers in microbiology · 2016Article
- Proteomic Analysis of the Hydrogen and Carbon Monoxide Metabolism of Methanothermobacter marburgensis.Frontiers in microbiology · 2016Article
- Energy Conservation Associated with Ethanol Formation from H2 and CO2 in Clostridium autoethanogenum Involving Electron Bifurcation.Journal of bacteriology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conversion of acetate to methane (aceticlastic methanogenesis) is an ecologically important process carried out exclusively by methanogenic archaea. An important enzyme for this process as well as for methanogenic growth on carbon monoxide is the five-subunit archaeal CO dehydrogenase/acetyl coenzyme A (CoA) synthase multienzyme complex (CODH/ACS) catalyzing both CO oxidation/CO(2) reduction and cleavage/synthesis of acetyl-CoA. Methanosarcina acetivorans C2A contains two very similar copies of a six-gene operon (cdh genes) encoding two isoforms of CODH/ACS (Cdh1 and Cdh2) and a single CdhA subunit, CdhA3. To address the role of the CODH/ACS system in M. acetivorans, mutational as well as promoter/reporter gene fusion analyses were conducted. Phenotypic characterization of cdh disruption mutants (three single and double mutants, as well as the triple mutant) revealed a strict requirement of either Cdh1 or Cdh2 for acetotrophic or carboxidotrophic growth, as well as for autotrophy, which demonstrated that both isoforms are bona fide CODH/ACS. While expression of the Cdh2-encoding genes was generally higher than that of genes encoding Cdh1, both appeared to be regulated differentially in response to growth phase and to changing substrate conditions. While dispensable for growth, CdhA3 clearly affected expression of cdh1, suggesting that it functions in signal perception and transduction rather than in catabolism. The data obtained argue for a functional hierarchy and regulatory cross talk of the CODH/ACS isoforms.
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Registered trials
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