ArticleEndocrinology2012
Identification, proliferation, and differentiation of adult Leydig stem cells.
Article in Endocrinology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
59 citing papers in PubMed.
- Genetic ablation of the adult Leydig cell lineage reveals stem Leydig cells and a peritubular regenerative niche in mice.Endocrinology · 2026Article
- The EV-mitochondrial outsourcing network as a therapeutic target for age-related testosterone deficiency: from network collapse to clinical intervention.Frontiers in endocrinology · 2026Review
- Subnutrition during gestation and lactation affects testicular development, serum testosterone concentration and androgen receptor expression in myoid cells of mature rats.Animal reproduction · 2026Article
- Enucleated bone marrow-derived mesenchymal stromal cells regulate immune microenvironment and promote testosterone production through efferocytosis.Reproductive biology and endocrinology : RB&E · 2025Article
- Exploring the Characters of Non-Coding RNAs in Spermatogenesis and Male Infertility.International journal of molecular sciences · 2025Review
- A New Leydig Cell-Exclusive Cre Line Allows Lineage Tracing of Fetal and Adult Leydig Cell Populations in the Mouse.Endocrinology · 2025Article
- Age-related testosterone decline: mechanisms and intervention strategies.Reproductive biology and endocrinology : RB&E · 2024Review
- Use ofFrontiers in toxicology · 2024Article
- Effects of aging and macrophages on mice stem Leydig cell proliferation and differentiationFrontiers in endocrinology · 2023Article
- Bone morphogenetic protein 4 inhibits rat stem/progenitor Leydig cell development and regeneration via SMAD-dependent and SMAD-independent signaling.Cell death & disease · 2022Article
- Testosterone promotion effect of Eucommia ulmoides staminate flower via the steroidogenic pathway and potential hormonal mechanism.Scientific reports · 2022Article
- In Vivo Skin Regeneration and Wound Healing Using Cell Micro-Transplantation.Pharmaceutics · 2022Article
- Behavior and Functional Roles of CD34International journal of molecular sciences · 2022Review
- Leptin secreted from testicular microenvironment modulates hedgehog signaling to augment the endogenous function of Leydig cells.Cell death & disease · 2022Article
- Sirt1 and Nrf2: regulation of Leydig cell oxidant/antioxidant intracellular environment and steroid formation†.Biology of reproduction · 2021Article
- Advances in stem cell research for the treatment of primary hypogonadism.Nature reviews. Urology · 2021Review
- TCF21Nature communications · 2021Article
- Effects of Midazolam on the Development of Adult Leydig Cells From Stem CellsFrontiers in endocrinology · 2021Article
- Androgen and Luteinizing Hormone Stimulate the Function of Rat Immature Leydig Cells Through Different Transcription Signals.Frontiers in endocrinology · 2021Article
- Neurotrophin-3 stimulates stem Leydig cell proliferation during regeneration in rats.Journal of cellular and molecular medicine · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Leydig cells, the testosterone-producing cells of the adult testis, rarely turn over. However, their elimination with ethane dimethanesulfonate (EDS) is followed by the appearance of new, fully functional adult Leydig cells. The cells that give rise to the new Leydig cells have not been well characterized, and little is known about the mechanism by which they are regulated. We isolated cells expressing platelet-derived growth factor receptor-α, but not 3β-hydroxysteroid dehydrogenase (3β-HSD(neg)) from the testes of EDS-treated adult rats. Depending on conditions, these cells proliferated indefinitely or differentiated and produced testosterone. To localize these cells and to determine the effect of the testicular environment on their function, the seminiferous tubules and testicular interstitium were physically separated and cultured. During the first 72 h in culture, 3β-HSD(neg) cells on the tubule surfaces underwent divisions. Some of these cells later expressed 3β-HSD and produced testosterone. Removal of the newly formed 3β-HSD(pos) cells from the tubule surfaces with EDS, followed by further culture of the stripped tubules, resulted in the reappearance of testosterone-producing cells. These results, taken together, suggest that the precursors for newly formed Leydig cells are stem cells, with many if not all situated on the surfaces of the seminiferous tubules. Although normally quiescent, the stem cells are capable of self-renewal and differentiation. The development of the tubule culture system should provide a valuable in vitro approach to assess the role(s) of niche components on the function of adult Leydig stem cells despite their residing in a complex mammalian tissue.
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Registered trials
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