ArticlePlant cell reports2012
5-Azacytidine combined with 2,4-D improves somatic embryogenesis of Acca sellowiana (O. Berg) Burret by means of changes in global DNA methylation levels.
Article in Plant cell reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 93 citations in OpenAlex.
- Targeting histone acetylation to enhance somatic embryogenesis in Quercus suber L.Tree physiology · 2025Article
- 5-AzaCytidine Promotes Somatic Embryogenesis ofPlants (Basel, Switzerland) · 2025Article
- Somatic embryogenesis from mature sorghum seeds: An underutilized genome editing recipient system.Heliyon · 2024Article
- Embryonic abnormalities and genotoxicity induced by 2,4-dichlorophenoxyacetic acid during indirect somatic embryogenesis in Coffea.Scientific reports · 2023Article
- Somaclonal Variation-Advantage or Disadvantage in Micropropagation of the Medicinal Plants.International journal of molecular sciences · 2023Review
- Genetic and epigenetic modes of the regulation of somatic embryogenesis: a review.Biologia futura · 2022Review
- Phenotypic variation and epigenetic insight into tissue culture berry crops.Frontiers in plant science · 2022Review
- Molecular Determinants ofFrontiers in plant science · 2022Review
- Epigenome and Epitranscriptome: Potential Resources for Crop Improvement.International journal of molecular sciences · 2021Review
- Review
- Comparative Study of the Genetic and Biochemical Variability ofInternational journal of molecular sciences · 2021Article
- Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture.Journal of biological research (Thessalonike, Greece) · 2021Article
- Tissue culture-induced DNA methylation in crop plants: a review.Molecular biology reports · 2021Review
- Induction of Radiata Pine Somatic Embryogenesis at High Temperatures Provokes a Long-Term Decrease in DNA Methylation/Hydroxymethylation and Differential Expression of Stress-Related Genes.Plants (Basel, Switzerland) · 2020Article
- A peak in global DNA methylation is a key step to initiate the somatic embryogenesis of coconut palm (Cocos nucifera L).Plant cell reports · 2020Article
- Hypermethylation of Auxin-Responsive Motifs in the Promoters of the Transcription Factor Genes Accompanies the Somatic Embryogenesis Induction in Arabidopsis.International journal of molecular sciences · 2020Article
- Epigenetic Regulation of Auxin-Induced Somatic Embryogenesis in Plants.International journal of molecular sciences · 2020Review
- Transcriptome profiling of 'Kyoho' grape at different stages of berry development following 5-azaC treatment.BMC genomics · 2019Article
- Long-Term Maintainable Somatic Embryogenesis System in Alfalfa (Plants (Basel, Switzerland) · 2019Article
- Somatic Embryogenesis Induction in Woody Species: The Future After OMICs Data Assessment.Frontiers in plant science · 2019Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledDNA methylation is an epigenetic regulatory mechanism of gene expression which can be associated with developmental phases and in vitro morphogenetic competence in plants. The present work evaluated the effects of 5-azacytidine (AzaC) and 2,4-dichlorophenoxyacetic acid (2,4-D) on Acca sellowiana somatic embryogenesis (SE) and global DNA methylation levels by high-performance liquid chromatography mass spectrometry (HPLC/MS/MS). 2,4-D-free treatments revealed no somatic embryo formation in both accessions tested. Treatments supplemented with 2,4-D pulse plus AzaC in the culture medium resulted in increased embryo formation. In AzaC-free treatment, HPLC/MS/MS analysis showed a gradual increase in methylation levels in cultures of both accessions tested during SE induction. Treatment with AzaC and 2,4-D-free resulted in a marked decrease in methylation for both accessions, ranging from 37.6 to 20.8 %. In treatment with 2,4-D and AzaC combined, the 85 accession showed increasing global methylation levels. Otherwise, the 101X458 accession, in the same treatment, showed a decrease between 10 and 20 days, followed by an increase after 30 days (39.5, 36.2 and 41.6 %). These results indicate that 2,4-D pulse combined with AzaC improves SE induction. However, the conversion phase showed that although positively influencing SE induction, AzaC had a dysregulatory effect on the stage of autotrophic plant formation, resulting in significantly lower conversion rates. The results suggest that DNA methylation dramatically influences SE in Acca sellowiana, and global DNA methylation dynamics are related to morphogenetic response. KEY MESSAGE: 5-Azacytidine combined with 2,4-D increases the number of Acca sellowiana somatic embryos. Global DNA methylation is directly affected by these compounds.
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