ArticleApplied and environmental microbiology2012
Engineering a cyanobacterial cell factory for production of lactic acid.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 188 citations in OpenAlex.
- Co-cultures of Microalgae and Other Microorganisms: Wastewater Treatment and Production of Value-Added Bioproducts.Applied biochemistry and biotechnology · 2026Review
- Isolation ofACS synthetic biology · 2025Article
- Cyanobacterial circadian regulation enhances bioproduction under subjective nighttime through rewiring of carbon partitioning dynamics, redox balance orchestration, and cell cycle modulation.Microbial cell factories · 2025Article
- Biotechnological Applications of Cyanobacteria: Synechocystis and Synechococcus Strains.Advances in biochemical engineering/biotechnology · 2025Review
- Cyanobacteria as cell factories for the photosynthetic production of sucrose.Frontiers in microbiology · 2023Review
- Metabolic Engineering Design Strategies for Increasing Carbon Fluxes Relevant for Biosynthesis in Cyanobacteria.Advances in biochemical engineering/biotechnology · 2023Article
- Light-Driven Synthetic Biology: Progress in Research and Industrialization of Cyanobacterial Cell Factory.Life (Basel, Switzerland) · 2022Review
- Heterologous Lactate Synthesis inApplied and environmental microbiology · 2022Article
- Expression and activity of heterologous hydroxyisocaproate dehydrogenases inEngineering microbiology · 2022Article
- Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis.Frontiers in bioengineering and biotechnology · 2022Review
- Channeling Anabolic Side Products toward the Production of Nonessential Metabolites: Stable Malate Production inACS synthetic biology · 2021Article
- Combinatorial assembly platform enabling engineering of genetically stable metabolic pathways in cyanobacteria.Nucleic acids research · 2021Article
- Comparison of alternative integration sites in the chromosome and the native plasmids of the cyanobacterium Synechocystis sp. PCC 6803 in respect to expression efficiency and copy number.Microbial cell factories · 2021Article
- Over-expression of an electron transport protein OmcS provides sufficient NADH for D-lactate production in cyanobacterium.Biotechnology for biofuels · 2021Article
- Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications.Genes · 2021Review
- Lactic acid production - producing microorganisms and substrates sources-state of art.Heliyon · 2020Review
- Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production.Communications biology · 2020Article
- Impact of RNase E and RNase J on Global mRNA Metabolism in the CyanobacteriumFrontiers in microbiology · 2020Article
- Preparation of cyanobacteria-enhanced poly(vinyl)alcohol-based films with resistance to blue-violet light / red light and water.PloS one · 2020Article
- Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic engineering of microorganisms has become a versatile tool to facilitate production of bulk chemicals, fuels, etc. Accordingly, CO(2) has been exploited via cyanobacterial metabolism as a sustainable carbon source of biofuel and bioplastic precursors. Here we extended these observations by showing that integration of an ldh gene from Bacillus subtilis (encoding an l-lactate dehydrogenase) into the genome of Synechocystis sp. strain PCC6803 leads to l-lactic acid production, a phenotype which is shown to be stable for prolonged batch culturing. Coexpression of a heterologous soluble transhydrogenase leads to an even higher lactate production rate and yield (lactic acid accumulating up to a several-millimolar concentration in the extracellular medium) than those for the single ldh mutant. The expression of a transhydrogenase alone, however, appears to be harmful to the cells, and a mutant carrying such a gene is rapidly outcompeted by a revertant(s) with a wild-type growth phenotype. Furthermore, our results indicate that the introduction of a lactate dehydrogenase rescues this phenotype by preventing the reversion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.