ArticleCellular and molecular neurobiology2013
Sealing of transected neurites of rat B104 cells requires a diacylglycerol PKC-dependent pathway and a PKA-dependent pathway.
Article in Cellular and molecular neurobiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Repair of traumatic lesions to the plasmalemma of neurons and other cells: Commonalities, conflicts, and controversies.Frontiers in physiology · 2023Review
- Mechanisms of Axon Elongation Following CNS Injury: What Is Happening at the Axon Tip?Frontiers in cellular neuroscience · 2020Article
- Role of axon resealing in retrograde neuronal death and regeneration after spinal cord injury.Neural regeneration research · 2019Review
- Structural and signaling role of lipids in plasma membrane repair.Current topics in membranes · 2019Review
- Cellular mechanisms and signals that coordinate plasma membrane repair.Cellular and molecular life sciences : CMLS · 2018Review
- Ca2+/calmodulin-dependent protein kinase II and Dimethyl Sulfoxide affect the sealing frequencies of transected hippocampal neurons.Journal of neuroscience research · 2018Article
- The Effect of Axon Resealing on Retrograde Neuronal Death after Spinal Cord Injury in Lamprey.Brain sciences · 2018Article
- Repair of traumatic plasmalemmal damage to neurons and other eukaryotic cells.Neural regeneration research · 2016Review
- Sealing frequency of B104 cells declines exponentially with decreasing transection distance from the axon hillock.Experimental neurology · 2016Article
- Effects of extracellular calcium and surgical techniques on restoration of axonal continuity by polyethylene glycol fusion following complete cut or crush severance of rat sciatic nerves.Journal of neuroscience research · 2016Article
- The curious ability of polyethylene glycol fusion technologies to restore lost behaviors after nerve severance.Journal of neuroscience research · 2016Review
- Mechanisms of neuronal membrane sealing following mechanical trauma.Neuroscience bulletin · 2014Review
- The large conductance Ca(2+) -activated K(+) (BKCa) channel regulates cell proliferation in SH-SY5Y neuroblastoma cells by activating the staurosporine-sensitive protein kinases.Frontiers in physiology · 2014Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
To survive, neurons and other eukaryotic cells must rapidly repair (seal) plasmalemmal damage. Such repair occurs by an accumulation of intracellular vesicles at or near the plasmalemmal disruption. Diacylglycerol (DAG)-dependent and cAMP-dependent proteins are involved in many vesicle trafficking pathways. Although recent studies have implicated the signaling molecule cAMP in sealing, no study has investigated how DAG and DAG-dependent proteins affect sealing. By means of dye exclusion to assess Ca(2+)-dependent vesicle-mediated sealing of transected neurites of individually identifiable rat hippocampal B104 cells, we now report that, compared to non-treated controls, sealing probabilities and rates are increased by DAG and cAMP analogs that activate PKC and Munc13-1 and PKA. Sealing is decreased by inhibiting DAG-activated novel protein kinase C isozymes η (nPKCη) and θ (nPKCθ) and Munc13-1, the PKC effector myristoylated alanine rich PKC substrate (MARCKS) or phospholipase C (PLC). DAG-increased sealing is prevented by inhibiting MARCKS or protein kinase A (PKA). Sealing probability is further decreased by simultaneously inhibiting nPKCη, nPKCθ, and PKA. Extracellular Ca(2+), DAG, or cAMP analogs do not affect this decrease in sealing. These and other data suggest that DAG increases sealing through MARCKS and that nPKCη, nPKCθ, and PKA are all required to seal plasmalemmal damage in B104 and likely all eukaryotic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.