ArticleNature protocols2012
Determining the polysaccharide composition of plant cell walls.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 193 papers.
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Who cites it
193 citing papers in PubMed.
- Trial
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- Rice straw sample preparation protocol for cell wall major constituent analysis via Raman spectroscopy.STAR protocols · 2026Article
- Structural Features of a Dancong Tea Polysaccharide and Its Amelioration of Glucolipid Metabolic Disorders Associated with Gut Microbiota Modulation.Foods (Basel, Switzerland) · 2026Article
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- Alginate foraging is conserved in geographically and taxonomically distinct ruminant microbiomes.Nature communications · 2026Article
- Monosaccharide Composition and In Vitro Lipid-Retention Properties of Ocimum basilicum L. Seed Mucilage.Chemistry & biodiversity · 2026Article
- Structural characterization, rheological properties, and skin bioactivities of a glucomannan from Bletilla striata.Natural products and bioprospecting · 2026Article
- The OsGSK1-OsBZR3-OsJAZ4 module integrates nitrate signaling with brassinosteroid and jasmonic acid crosstalk to drive cell wall remodeling for salt recovery in rice.Plant communications · 2026Article
- Enzymatic Biocontrol of Fire Blight (Environmental science & technology · 2026Article
- An oral immune enhancer with excellent biocompatibility for effectively strengthening body's immunity and treating cancer.Acta pharmaceutica Sinica. B · 2026Article
- Sugarcane streak mosaic virus P1 protein targets the BES1 transcription factor to subvert DAMP-triggered immunity in plant.Science advances · 2026Article
- Optimized Alkaline Hydrolysis for Recovering Ferulated Arabinoxylan Biopolymers from Maize Bran with Antioxidant Functionality.Polymers · 2026Article
- Intrapolysaccharides from Amazonian Panus lecomtei induce pro-inflammatory responses and adhere to macrophage surfaces.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Microbial Biofilms Dynamics and Functionality in an UrbanEnvironmental science & technology · 2026Article
- Isolation and purification of polysaccharides from Centella Asiatica followed by structural characterization and evaluation of antidiabetic activity.Scientific reports · 2026Article
- Genetic Interaction Between Bph50 and Bph51 Confers Resistance to Brown Planthopper in Rice.Rice (New York, N.Y.) · 2026Article
- Arabinoxylan and pectin features differentiate maize inbred lines with contrasting Fusarium stalk rot resistance.Frontiers in plant science · 2026Article
133 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant cell wall is a chemically complex structure composed mostly of polysaccharides. Detailed analyses of these cell wall polysaccharides are essential for our understanding of plant development and for our use of plant biomass (largely wall material) in the food, agriculture, fabric, timber, biofuel and biocomposite industries. We present analytical techniques not only to define the fine chemical structures of individual cell wall polysaccharides but also to estimate the overall polysaccharide composition of cell wall preparations. The procedure covers the preparation of cell walls, together with gas chromatography-mass spectrometry (GC-MS)-based methods, for both the analysis of monosaccharides as their volatile alditol acetate derivatives and for methylation analysis to determine linkage positions between monosaccharide residues as their volatile partially methylated alditol acetate derivatives. Analysis time will vary depending on both the method used and the tissue type, and ranges from 2 d for a simple neutral sugar composition to 2 weeks for a carboxyl reduction/methylation linkage analysis.
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