ArticleThe EMBO journal2012
CSR-1 RNAi pathway positively regulates histone expression in C. elegans.
Article in The EMBO journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- SLBP-independent control of maternal histone mRNA.bioRxiv : the preprint server for biology · 2026Article
- Germ granule localization of nematode Argonaute WAGO-4 ensures fidelity in small RNA loading.The EMBO journal · 2025Article
- Regulation of MORC-1 is key to the CSR-1-mediated germline gene licensing mechanism inScience advances · 2025Article
- FG repeats drive co-clustering of nuclear pores and P granules in the C. elegans germline.Development (Cambridge, England) · 2025Article
- Requirement of CSR-1 isoforms and catalytic activity formicroPublication biology · 2025Article
- Argonaute protein CSR-1 restricts localization of holocentromere protein HCP-3, the C. elegans CENP-A homolog.Journal of cell science · 2024Article
- Catalytic residues of microRNA Argonautes play a modest role in microRNA star strand destabilization in C. elegans.Nucleic acids research · 2024Article
- A role for the C. elegans Argonaute protein CSR-1 in small nuclear RNA 3' processing.PLoS genetics · 2024Article
- The catalytic activity of microRNA Argonautes plays a modest role in microRNA star strand destabilization inbioRxiv : the preprint server for biology · 2024Article
- Coordinated expression of replication-dependent histone genes from multiple loci promotes histone homeostasis inMolecular biology of the cell · 2023Article
- Epigenetic dynamics during germline development: insights from Drosophila and C. elegans.Current opinion in genetics & development · 2023Review
- A family of C. elegans VASA homologs control Argonaute pathway specificity and promote transgenerational silencing.Cell reports · 2022Article
- Article
- piRNAs coordinate poly(UG) tailing to prevent aberrant and perpetual gene silencing.Current biology : CB · 2021Article
- Two isoforms of the essential C. elegans Argonaute CSR-1 differentially regulate sperm and oocyte fertility.Nucleic acids research · 2021Article
- Arginine methylation promotes siRNA-binding specificity for a spermatogenesis-specific isoform of the Argonaute protein CSR-1.Nature communications · 2021Article
- Small RNAs and chromatin in the multigenerational epigenetic landscape ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Germline inherited small RNAs facilitate the clearance of untranslated maternal mRNAs in C. elegans embryos.Nature communications · 2021Article
- mRNA Editing, Processing and Quality Control inGenetics · 2020Review
- Widespread roles for piRNAs and WAGO-class siRNAs in shaping the germline transcriptome of Caenorhabditis elegans.Nucleic acids research · 2020Article
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Abstract
Endogenous small interfering RNAs (endo-siRNAs) have been discovered in many organisms, including mammals. In C. elegans, depletion of germline-enriched endo-siRNAs found in complex with the CSR-1 Argonaute protein causes sterility and defects in chromosome segregation in early embryos. We discovered that knockdown of either csr-1, the RNA-dependent RNA polymerase (RdRP) ego-1, or the dicer-related helicase drh-3, leads to defects in histone mRNA processing, resulting in severe depletion of core histone proteins. The maturation of replication-dependent histone mRNAs, unlike that of other mRNAs, requires processing of their 3'UTRs through an endonucleolytic cleavage guided by the U7 snRNA, which is lacking in C. elegans. We found that CSR-1-bound antisense endo-siRNAs match histone mRNAs and mRNA precursors. Consistently, we demonstrate that CSR-1 directly binds to histone mRNA in an ego-1-dependent manner using biotinylated 2'-O-methyl RNA oligonucleotides. Moreover, we demonstrate that increasing the dosage of histone genes rescues the lethality associated with depletion of CSR-1 and EGO-1. These results support a positive and direct effect of RNAi on histone gene expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.