ArticleEpigenetics2012
Transgenerational maintenance of transgene body CG but not CHG and CHH methylation.
Article in Epigenetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- DNA Methylation Is Crucial for 1-Methylcyclopropene Delaying Postharvest Ripening and Senescence of Tomato Fruit.International journal of molecular sciences · 2024Article
- Association of DNA methylation and transcriptome reveals epigenetic etiology of heart failure.Functional & integrative genomics · 2022Article
- Deciphering the Epigenetic Alphabet Involved in Transgenerational Stress Memory in Crops.International journal of molecular sciences · 2021Review
- Review
- Elevated COScientific reports · 2020Article
- Small DNA Methylation, Big Player in Plant Abiotic Stress Responses and Memory.Frontiers in plant science · 2020Review
- The Rapid Methylation of T-DNAs UponFrontiers in plant science · 2019Article
- Comparative Analysis of DNA Methylation Reveals Specific Regulations on Ethylene Pathway in Tomato Fruit.Genes · 2018Article
- Genome-wide DNA Methylation analysis in response to salinity in the model plant caliph medic (Medicago truncatula).BMC genomics · 2018Article
- Impacts of elevated COScientific reports · 2017Article
- Genome-wide identification of endogenous RNA-directed DNA methylation loci associated with abundant 21-nucleotide siRNAs in Arabidopsis.Scientific reports · 2016Article
- Stable Epigenetic Variants Selected from an Induced HypomethylatedFrontiers in plant science · 2016Article
- A preliminary exploration on DNA methylation of transgene across generations in transgenic rats.Scientific reports · 2015Article
- Replicating Potato spindle tuber viroid mediates de novo methylation of an intronic viroid sequence but no cleavage of the corresponding pre-mRNA.RNA biology · 2015Article
- Epigenetic variation, inheritance, and parent-of-origin effects of cytosine methylation in maize (Zea mays).Genetics · 2014Article
- An endogene-resembling transgene is resistant to DNA methylation and systemic silencing.RNA biology · 2014Article
- Roles, and establishment, maintenance and erasing of the epigenetic cytosine methylation marks in plants.Journal of genetics · 2013Review
- Progressive 35S promoter methylation increases rapidly during vegetative development in transgenic Nicotiana attenuata plants.BMC plant biology · 2013Article
- Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture.Epigenetics · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, RNA-directed DNA methylation (RdDM) can target both transgene promoters and coding regions/gene bodies. RdDM leads to methylation of cytosines in all sequence contexts: CG, CHG and CHH. Upon segregation of the RdDM trigger, at least CG methylation can be maintained at promoter regions in the progeny. So far, it is not clear whether coding region methylation can be also maintained. We showed that the body of Potato spindle tuber viroid (PSTVd) transgene constructs became densely de novo methylated at CG, CHG and CHH sites upon PSTVd infection. In this study, we demonstrate that in viroid-free progeny plants, asymmetric CHH and CHG methylation was completely lost. However, symmetric CG methylation was stably maintained for at least two generations. Importantly, the presence of transgene body methylation did not lead to an increase of dimethylation of histone H3 lysine 9 or a decrease of acetylation of H3. Our data supports the view that CG methylation can be maintained not only in promoters but also in the body of transgenes. They further suggest that maintenance of methylation may occur independently of tested chromatin modifications.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.