ArticleCell & bioscience2012
Oocyte-like cells induced from mouse spermatogonial stem cells.
Article in Cell & bioscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Pooled it
- Establishment of a Spermatogonial Stem Cell Line with Potential of Meiosis in a Hermaphroditic Fish,Cells · 2022Article
- Identification of Stem Cell-Like Cells in the Ovary.Tissue engineering and regenerative medicine · 2022Review
- Article
- Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.Stem cell research & therapy · 2019Article
- Review
- Article
- Differentiation of early germ cells from human skin-derived stem cells without exogenous gene integration.Scientific reports · 2015Article
- PTEN signaling is required for the maintenance of spermatogonial stem cells in mouse, by regulating the expressions of PLZF and UTF1.Cell & bioscience · 2015Article
- Connexin43 is required for the maintenance of multipotency in skin-derived stem cells.Stem cells and development · 2014Article
- Induced pluripotent stem cell potential in medicine, specifically focused on reproductive medicine.Frontiers in surgery · 2014Review
- Long exposure to mature ooplasm can alter DNA methylation at imprinted loci in non-growing oocytes but not in prospermatogonia.Reproduction (Cambridge, England) · 2014Article
- Differentiation of newborn mouse skin derived stem cells into germ-like cells in vitro.Journal of visualized experiments : JoVE · 2013Article
- The 2012 Ming K Jeang award for excellence in cell & bioscience.Cell & bioscience · 2013Article
- Plasticity of spermatogonial stem cells.Asian journal of andrologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelled
backgroundDuring normal development primordial germ cells (PGCs) derived from the epiblast are the precursors of spermatogonia and oogonia. In culture, PGCs can be induced to dedifferentiate to pluripotent embryonic germ (EG) cells in the presence of various growth factors. Several recent studies have now demonstrated that spermatogonial stem cells (SSCs) can also revert back to pluripotency as embryonic stem (ES)-like cells under certain culture conditions. However, the potential dedifferentiation of SSCs into PGCs or the potential generation of oocytes from SSCs has not been demonstrated before.
resultsWe report that mouse male SSCs can be converted into oocyte-like cells in culture. These SSCs-derived oocytes (SSC-Oocs) were similar in size to normal mouse mature oocytes. They expressed oocyte-specific markers and gave rise to embryos through parthenogenesis. Interestingly, the Y- and X-linked testis-specific genes in these SSC-Oocs were significantly down-regulated or turned off, while oocyte-specific X-linked genes were activated. The gene expression profile appeared to switch to that of the oocyte across the X chromosome. Furthermore, these oocyte-like cells lost paternal imprinting but acquired maternal imprinting.
conclusionsOur data demonstrate that SSCs might maintain the potential to be reprogrammed into oocytes with corresponding epigenetic reversals. This study provides not only further evidence for the remarkable plasticity of SSCs but also a potential system for dissecting molecular and epigenetic regulations in germ cell fate determination and imprinting establishment during gametogenesis.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.