ArticleBMC plant biology2012
High throughput sequencing reveals novel and abiotic stress-regulated microRNAs in the inflorescences of rice.
Article in BMC plant biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
75 citing papers in PubMed, 1 synthesis or guideline pooled it, 179 citations in OpenAlex.
- The role of microRNAs in responses to drought and heat stress in peanut (Arachis hypogaea).The plant genome · 2023Pooled it
- Identification of Freezing-Responsive microRNAs and Their Targets in Chinese Jujube by Small RNA and Degradome Sequencing.International journal of molecular sciences · 2026Article
- Identification of miRNAs in Response to Cold Stress in 'Chaling' Common Wild Rice (Life (Basel, Switzerland) · 2025Article
- ThePlants (Basel, Switzerland) · 2025Article
- Small Interfering RNAs as Critical Regulators of Plant Life Process: New Perspectives on Regulating the Transcriptomic Machinery.International journal of molecular sciences · 2025Review
- Identifying long non-coding RNAs involved in heat stress response during wheat pollen development.Frontiers in plant science · 2024Article
- MicroRNA2871b of Dongxiang Wild Rice (International journal of molecular sciences · 2023Article
- Integration of miRNA dynamics and drought tolerant QTLs in rice reveals the role of miR2919 in drought stress response.BMC genomics · 2023Article
- Comparative Genome-Wide Analysis of MicroRNAs and Their Target Genes in Roots of ContrastingGenes · 2023Article
- Miniature Inverted-Repeat Transposable Elements: Small DNA Transposons That Have Contributed to PlantPlants (Basel, Switzerland) · 2023Review
- Tapping into the plasticity of plant architecture for increased stress resilience.F1000Research · 2023Review
- Research progress on plant noncoding RNAs in response to low-temperature stress.Plant signaling & behavior · 2022Review
- Review
- Article
- Integrated Multi-Omics Perspective to Strengthen the Understanding of Salt Tolerance in Rice.International journal of molecular sciences · 2022Review
- Molecular insights into mechanisms underlying thermo-tolerance in tomato.Frontiers in plant science · 2022Review
- Genomics and transcriptomics to protect rice (Frontiers in plant science · 2022Review
- Extracellular vesicles derived fromFrontiers in immunology · 2022Article
- Genome-wide in silico analysis of long intergenic non-coding RNAs from rice peduncles at the heading stage.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Tuning Beforehand: A Foresight on RNA Interference (RNAi) and In Vitro-Derived dsRNAs to Enhance Crop Resilience to Biotic and Abiotic Stresses.International journal of molecular sciences · 2021Review
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundMicroRNAs (miRNAs) are small RNA molecules that play important regulatory roles in plant development and stress responses. Identification of stress-regulated miRNAs is crucial for understanding how plants respond to environmental stimuli. Abiotic stresses are one of the major factors that limit crop growth and yield. Whereas abiotic stress-regulated miRNAs have been identified in vegetative tissues in several plants, they are not well studied in reproductive tissues such as inflorescences.
resultsWe used Illumina deep sequencing technology to sequence four small RNA libraries that were constructed from the inflorescences of rice plants that were grown under control condition and drought, cold, or salt stress. We identified 227 miRNAs that belong to 127 families, including 70 miRNAs that are not present in the miRBase. We validated 62 miRNAs (including 10 novel miRNAs) using published small RNA expression data in DCL1, DCL3, and RDR2 RNAi lines and confirmed 210 targets from 86 miRNAs using published degradome data. By comparing the expression levels of miRNAs, we identified 18, 15, and 10 miRNAs that were regulated by drought, cold and salt stress conditions, respectively. In addition, we identified 80 candidate miRNAs that originated from transposable elements or repeats, especially miniature inverted-repeat elements (MITEs).
conclusionWe discovered novel miRNAs and stress-regulated miRNAs that may play critical roles in stress response in rice inflorescences. Transposable elements or repeats, especially MITEs, are rich sources for miRNA origination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.