ArticleBritish journal of pharmacology2012
Functional and morphological properties of pericytes in suburothelial venules of the mouse bladder.
Article in British journal of pharmacology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Role of ANO1 CaPflugers Archiv : European journal of physiology · 2025Article
- Intramural Blood Vessels as a Primary Site of Vascular LUTS.Lower urinary tract symptoms · 2025Review
- A multiscale vascular atlas of blood vessels supplying the urinary bladder of male and female mice.American journal of physiology. Renal physiology · 2025Article
- An endothelial-related prognostic index for bladder cancer patients.Discover oncology · 2024Article
- A positive association between the prevalence of circadian syndrome and overactive bladder in United States adults.Frontiers in public health · 2023Article
- Identification of endothelial-related molecular subtypes for bladder cancer patients.Frontiers in oncology · 2023Article
- The dynamic roles of the bladder tumour microenvironment.Nature reviews. Urology · 2022Review
- A Method to Isolate Pericytes From the Mouse Urinary Bladder for the Study of Diabetic Bladder Dysfunction.International neurourology journal · 2020Article
- Neural regulation of the contractility of nutrient artery in the guinea pig tibia.Pflugers Archiv : European journal of physiology · 2020Article
- Excitability and contractility in arterioles and venules from the urinary bladder.Current topics in membranes · 2020Review
- Synchrony of spontaneous CaJournal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2020Review
- Role of capillary pericytes in the integration of spontaneous CaThe Journal of physiology · 2018Article
- Contractile properties of periosteal arterioles in the guinea-pig tibia.Pflugers Archiv : European journal of physiology · 2017Article
- Properties of synchronous spontaneous CaPflugers Archiv : European journal of physiology · 2017Article
- Role of mucosa in generating spontaneous activity in the guinea pig seminal vesicle.The Journal of physiology · 2017Article
- The link between vascular dysfunction, bladder ischemia, and aging bladder dysfunction.Therapeutic advances in urology · 2017Review
- Mechanisms underlying spontaneous constrictions of postcapillary venules in the rat stomach.Pflugers Archiv : European journal of physiology · 2016Article
- Spontaneous activity in the microvasculature of visceral organs: role of pericytes and voltage-dependent Ca(2+) channels.The Journal of physiology · 2016Review
- Are blood vessels a target to treat lower urinary tract dysfunction?Naunyn-Schmiedeberg's archives of pharmacology · 2015Article
- Functional properties of submucosal venules in the rat stomach.Pflugers Archiv : European journal of physiology · 2015Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background and purposeIn suburothelial venules of rat bladder, pericytes (perivascular cells) develop spontaneous Ca(2+) transients, which may drive the smooth muscle wall to generate spontaneous venular constrictions. We aimed to further explore the morphological and functional characteristics of pericytes in the mouse bladder. EXPERIMENTAL APPROACH: The morphological features of pericytes were investigated by electron microscopy and fluorescence immunohistochemistry. Changes in diameters of suburothelial venules were measured using video microscopy, while intracellular Ca(2+) dynamics were visualized using Fluo-4 fluorescence Ca(2+) imaging. KEY
resultsA network of α-smooth muscle actin immunoreactive pericytes surrounded venules in the mouse bladder suburothelium. Scanning electron microscopy revealed that this network of stellate-shaped pericytes covered the venules, while transmission electron microscopy demonstrated that the venular wall consisted of endothelium and adjacent pericytes, lacking an intermediate smooth muscle layer. Pericytes exhibited spontaneous Ca(2+) transients, which were accompanied by phasic venular constrictions. Nicardipine (1 μM) disrupted the synchrony of spontaneous Ca(2+) transients in pericytes and reduced their associated constrictions. Residual asynchronous Ca(2+) transients were suppressed by cyclopiazonic acid (10 μM), 2-aminoethoxydiphenyl borate (10 μM), U-73122 (1 μM), oligomycin (1 μM) and SKF96365 (10 μM), but unaffected by ryanodine (100 μM) or YM-244769 (1 μM), suggesting that pericyte Ca(2+) transients rely on Ca(2+) release from the endoplasmic reticulum via the InsP(3) receptor and also require Ca(2+) influx through store-operated Ca(2+) channels. CONCLUSIONS AND IMPLICATIONS: The pericytes in mouse bladder can generate spontaneous Ca(2+) transients and contractions, and thus have a fundamental role in promoting spontaneous constrictions of suburothelial venules.
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