ArticleMicrocirculation (New York, N.Y. : 1994)2012
Coordination of intercellular Ca(2+) signaling in endothelial cell tubes of mouse resistance arteries.
Article in Microcirculation (New York, N.Y. : 1994), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 30 citations in OpenAlex.
- Guidelines for evaluating endothelial function in vascular tissue.American journal of physiology. Heart and circulatory physiology · 2026Review
- The host calcium system contributes to intracellularInfection and immunity · 2025Article
- Cracking the Endothelial Calcium (CaInternational journal of molecular sciences · 2023Review
- Mitochondrial ATP Production is Required for Endothelial Cell Control of Vascular Tone.Function (Oxford, England) · 2023Article
- Endothelial Ion Channels and Cell-Cell Communication in the Microcirculation.Frontiers in physiology · 2022Review
- Carbenoxolone and 18β-glycyrrhetinic acid inhibit inositol 1,4,5-trisphosphate-mediated endothelial cell calcium signalling and depolarise mitochondria.British journal of pharmacology · 2021Article
- Calcium-Dependent Ion Channels and the Regulation of Arteriolar Myogenic Tone.Frontiers in physiology · 2021Review
- Disrupted endothelial cell heterogeneity and network organization impair vascular function in prediabetic obesity.Metabolism: clinical and experimental · 2020Article
- Modeling the role of endoplasmic reticulum-mitochondria microdomains in calcium dynamics.Scientific reports · 2019Article
- Heterogeneity and emergent behaviour in the vascular endothelium.Current opinion in pharmacology · 2019Review
- Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium.Journal of visualized experiments : JoVE · 2019Article
- Calcium and electrical dynamics in lymphatic endothelium.The Journal of physiology · 2017Article
- Impact of Aging on Calcium Signaling and Membrane Potential in Endothelium of Resistance Arteries: A Role for Mitochondria.The journals of gerontology. Series A, Biological sciences and medical sciences · 2017Article
- The Endothelium Solves Problems That Endothelial Cells Do Not Know Exist.Trends in pharmacological sciences · 2017Review
- Calcium and electrical signaling in arterial endothelial tubes: New insights into cellular physiology and cardiovascular function.Microcirculation (New York, N.Y. : 1994) · 2017Review
- Acetylcholine released by endothelial cells facilitates flow-mediated dilatation.The Journal of physiology · 2016Article
- Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Endothelial mitochondria regulate the intracellular Ca2+ response to fluid shear stress.American journal of physiology. Cell physiology · 2016Article
- Advancing Age Decreases Pressure-Sensitive Modulation of Calcium Signaling in the Endothelium of Intact and Pressurized Arteries.Journal of vascular research · 2016Article
- Pressure-dependent regulation of Ca2+ signalling in the vascular endothelium.The Journal of physiology · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
objectiveTo test the hypothesis that Ca(2+) responses to GPCR activation are coordinated between neighboring ECs of resistance arteries.
methodsEC tubes were freshly isolated from superior epigastric arteries of C57BL/6 mice. Intercellular coupling was tested using microinjection of propidium iodide. Following loading with fluo-4 dye, intracellular Ca(2+) responses to ACh were imaged with confocal microscopy.
resultsCell-to-cell transfer of propidium iodide confirmed functional GJCs. A 1 μm ACh stimulus evoked Ca(2+) responses (9.8 ± 0.8/min, F/F(0) = 3.11 ± 0.2) which pseudo-line-scan analysis revealed as composed of Ca(2+) waves and spatially restricted Ca(2+) release events. A 100 nm ACh stimulus induced Ca(2+) responses of lower frequency (4.5 ± 0.7/min) and amplitude (F/F(0) = 1.95 ± 0.11) composed primarily of spatially restricted events. The time interval between Ca(2+) waves in adjacent cells (0.79 ± 0.12 s) was shorter (p < 0.05) than that between nonadjacent cells (1.56 ± 0.25 s). Spatially restricted Ca(2+) release events had similar frequencies and latencies between adjacent and nonadjacent cells. Inhibiting intracellular Ca(2+) release with 2-APB, Xestospongin C or thapsigargin eliminated Ca(2+) responses.
conclusionsWith moderate GPCR stimulation, localized Ca(2+) release events predominate among cells. Greater GPCR stimulation evokes coordinated intercellular Ca(2+) waves via the ER. Calcium signaling during GPCR activation is complex among cells, varying with stimulus intensity and proximity to actively signaling cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.