ArticlePloS one2012
Derivation of corneal endothelial cell-like cells from rat neural crest cells in vitro.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- Strategies for Differentiation of Corneal Endothelial Cells From Stem Cells: Current Advances and Challenges.Stem cells international · 2026Review
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- Transforming meat waste into sustainable corneal keratoplasty models.Frontiers in bioengineering and biotechnology · 2025Article
- Focus on seed cells: stem cells in 3D bioprinting of corneal grafts.Frontiers in bioengineering and biotechnology · 2024Review
- Biomaterials used for tissue engineering of barrier-forming cell monolayers in the eye.Frontiers in bioengineering and biotechnology · 2023Review
- Pluripotent stem cell-derived corneal endothelial cells as an alternative to donor corneal endothelium in keratoplasty.Stem cell reports · 2021Article
- Experimental design of a culture approach for corneal endothelial cells of New Zealand white rabbit.Heliyon · 2020Article
- Neural crest stem cells from human epidermis of aged donors maintain their multipotency in vitro and in vivo.Scientific reports · 2019Article
- Transcription factor TFAP2B up-regulates human corneal endothelial cell-specific genes during corneal development and maintenance.The Journal of biological chemistry · 2019Article
- Construction of a full-thickness human corneal substitute from anterior acellular porcine corneal matrix and human corneal cells.International journal of ophthalmology · 2019Article
- Article
- Promoting the expansion and function of human corneal endothelial cells with an orbital adipose-derived stem cell-conditioned medium.Stem cell research & therapy · 2017Article
- Therapy of corneal endothelial dysfunction with corneal endothelial cell-like cells derived from skin-derived precursors.Scientific reports · 2017Article
- Concise Review: Stem Cells for Corneal Wound Healing.Stem cells (Dayton, Ohio) · 2017Review
- Skin-Derived Precursors as a Source of Progenitors for Corneal Endothelial Regeneration.Stem cells translational medicine · 2017Article
- Prospect of Human Pluripotent Stem Cell-Derived Neural Crest Stem Cells in Clinical Application.Stem cells international · 2016Review
- Three-Dimensional Construction of a Rabbit Anterior Corneal Replacement for Lamellar Keratoplasty.PloS one · 2016Article
- Substrates for Expansion of Corneal Endothelial Cells towards Bioengineering of Human Corneal Endothelium.Journal of functional biomaterials · 2015Review
- Treatment with retinoic acid and lens epithelial cell-conditioned mediumExperimental and therapeutic medicine · 2015Article
- Isolation and transplantation of corneal endothelial cell-like cells derived from in-vitro-differentiated human embryonic stem cells.Stem cells and development · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to investigate the feasibility of inducing rat neural crest cells (NCC) to differentiate to functional corneal endothelial cell (CEC)-like cells in vitro. Rat NCC were induced with adult CEC-derived conditioned medium. Immunofluorescence, flow cytometry and real time RT-PCR assay were used to detect expression of the corneal endothelium differentiation marker N-cadherin and transcription factors FoxC1 and Pitx2. CFDA SE-labeled CEC-like cells were transplanted to the corneal endothelium of a rat corneal endothelium deficiency model, and an eye-down position was maintained for 24 hours to allow cell attachment. The animals were observed for as long as 2 months after surgery and underwent clinical and histological examination. Spindle-like NCC turned to polygonal CEC-like after induction and expressed N-cadherin, FoxC1, Pitx2, zonula occludens-1 and sodium-potassium pump Na(+)/K(+) ATPase. The corneas of the experimental group were much clearer than those of the control group and the mean corneal thickness in the experimental group was significantly less than in the control group7, 14, 21 and 28 days after surgery. Confocal microscopy through focusing and histological analysis confirmed that green fluorescence-positive CEC-like cells formed a monolayer covering the Descemet's membrane in the experimental group. In conclusion, CEC-like cells derived from NCCs displayed characters of native CEC, and the induction protocol provides guidance for future human CEC induction from NCC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.