ArticlePloS one2012
De novo sequencing of Hypericum perforatum transcriptome to identify potential genes involved in the biosynthesis of active metabolites.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Inspired by taxol: biosynthesis and engineering of hypericin and other phytochemicals in Hypericum.Planta · 2026Review
- Single molecule real-time sequencing data sets of Hypericum perforatum L. plantlets and cell suspension cultures.Scientific data · 2024Article
- Comparative transcriptome among Euscaphis konishii Hayata tissues and analysis of genes involved in flavonoid biosynthesis and accumulation.BMC genomics · 2019Article
- Comparative Transcriptome Analysis Identifies Genes Involved in Diosgenin Biosynthesis inMolecules (Basel, Switzerland) · 2019Article
- Pistil Transcriptome Analysis to Disclose Genes and Gene Products Related to Aposporous Apomixis inFrontiers in plant science · 2017Article
- Characterization of the Lycium barbarum fruit transcriptome and development of EST-SSR markers.PloS one · 2017Article
- Transcriptional Responses of Chilean Quinoa (Frontiers in plant science · 2017Article
- Transcriptome analysis revealed the dynamic oil accumulation in Symplocos paniculata fruit.BMC genomics · 2016Article
- Review
- Melatonin in Plants - Diversity of Levels and Multiplicity of Functions.Frontiers in plant science · 2016Review
- A Perspective on Hypericum perforatum Genetic Transformation.Frontiers in plant science · 2016Review
- Comparative Transcriptome Reconstruction of Four Hypericum Species Focused on Hypericin Biosynthesis.Frontiers in plant science · 2016Article
- Removal of redundant contigs from de novo RNA-Seq assemblies via homology search improves accurate detection of differentially expressed genes.BMC genomics · 2015Article
- Andrographis paniculata transcriptome provides molecular insights into tissue-specific accumulation of medicinal diterpenes.BMC genomics · 2015Article
- De novo sequencing of the Hypericum perforatum L. flower transcriptome to identify potential genes that are related to plant reproduction sensu lato.BMC genomics · 2015Article
- De Novo Assembly and Transcriptome Characterization of Canine Retina Using High-Throughput Sequencing.Genetics research international · 2015Article
- Article
- Transcriptome sequencing of Codonopsis pilosula and identification of candidate genes involved in polysaccharide biosynthesis.PloS one · 2015Article
- An insight into the genes involved in secoiridoid biosynthesis in Gentiana macrophylla by RNA-seq.Molecular biology reports · 2014Article
- Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHypericum perforatum L. (St. John's wort) is a medicinal plant with pharmacological properties that are antidepressant, anti-inflammatory, antiviral, anti-cancer, and antibacterial. Its major active metabolites are hypericins, hyperforins, and melatonin. However, little genetic information is available for this species, especially that concerning the biosynthetic pathways for active ingredients. METHODOLOGY/PRINCIPAL
findingsUsing de novo transcriptome analysis, we obtained 59,184 unigenes covering the entire life cycle of these plants. In all, 40,813 unigenes (68.86%) were annotated and 2,359 were assigned to secondary metabolic pathways. Among them, 260 unigenes are involved in the production of hypericin, hyperforin, and melatonin. Another 2,291 unigenes are classified as potential Type III polyketide synthase. Our BlastX search against the AGRIS database reveals 1,772 unigenes that are homologous to 47 known Arabidopsis transcription factor families. Further analysis shows that 10.61% (6,277) of these unigenes contain 7,643 SSRs.
conclusionWe have identified a set of putative genes involved in several secondary metabolism pathways, especially those related to the synthesis of its active ingredients. Our results will serve as an important platform for public information about gene expression, genomics, and functional genomics in H. perforatum.
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