ArticlePloS one2012
Proteomic analysis of PKCγ-related proteins in the spinal cord of morphine-tolerant rats.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 12 citations in OpenAlex.
- Circular RNA expression profile in the spinal cord of morphine tolerated rats and screen of putative key circRNAs.Molecular brain · 2019Article
- miR-873a-5p Targets A20 to Facilitate Morphine Tolerance in Mice.Frontiers in neuroscience · 2019Article
- Identification of lncRNA expression profiles and ceRNA analysis in the spinal cord of morphine-tolerant rats.Molecular brain · 2018Article
- The Proteomics of Intrathecal Analgesic agents for Chronic Pain.Current neuropharmacology · 2017Review
- Cypermethrin-induced nigrostriatal dopaminergic neurodegeneration alters the mitochondrial function: a proteomics study.Molecular neurobiology · 2015Article
- Proteomic approaches and identification of novel therapeutic targets for alcoholism.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Review
- An integrated quantitative proteomics and systems biology approach to explore synaptic protein profile changes during morphine exposure.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Review
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMorphine tolerance is a common drawback of chronic morphine exposure, hindering use of this drug. Studies have shown that PKCã may play a key role in the development of morphine tolerance, although the mechanisms are not fully known. METHODOLOGY/PRINCIPAL
findingsIn a rat model of morphine tolerance, PKCã knockdown in the spinal cord was successfully carried out using RNA interference (RNAi) with lentiviral vector-mediated short hairpin RNA of PKCã (LV-shPKCã). Spinal cords (L4-L5) were obtained surgically from morphine-tolerant (MT) rats with and without PKCã knockdown, for comparative proteomic analysis. Total proteins from the spinal cords (L4-L5) were extracted and separated using two-dimensional gel electrophoresis (2DGE); 2D gel images were analyzed with PDQuest software. Seven differential gel-spots were observed with increased spot volume, and 18 spots observed with decreased spot volume. Among these, 13 differentially expressed proteins (DEPs) were identified with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS), comparing between MT rats with and without PKCã knockdown. The DEPs identified have roles in the cytoskeleton, as neurotrophic factors, in oxidative stress, in ion metabolism, in cell signaling, and as chaperones. Three DEPs (GFAP, FSCN and GDNF) were validated with Western blot analysis, confirming the DEP data. Furthermore, using immunohistochemical analysis, we reveal for the first time that FSCN is involved in the development of morphine tolerance. CONCLUSIONS/SIGNIFICANCE: These data cast light on the proteins associated with the PKCã activity during morphine tolerance, and hence may contribute to clarification of the mechanisms by which PKCã influences MT.
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