ArticlePloS one2012
Cathepsin K-Cre causes unexpected germline deletion of genes in mice.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Germline-mediated ubiquitous recombination in ScxCre male mice: implications for tendon research.bioRxiv : the preprint server for biology · 2026Article
- Germline-mediated ubiquitous recombination in ScxCre male mice: Implications for tendon research.PloS one · 2026Article
- The Periosteum in Health and Disease.Current osteoporosis reports · 2025Review
- Loss of Brd4 alleviates pathological bone loss via Slc9b2 suppression in osteoclastogenesis.Clinical and translational medicine · 2025Article
- Minimally invasive longitudinal intravital imaging of cellular dynamics in intact long bone.Nature protocols · 2023Review
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- JAK2-IGF1 axis in osteoclasts regulates postnatal growth in mice.JCI insight · 2021Article
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- Hdac3 regulates bone modeling by suppressing osteoclast responsiveness to RANKL.The Journal of biological chemistry · 2020Article
- Conditional Knockout of PKC-δ in Osteoclasts Favors Bone Mass Accrual in Males Due to Decreased Osteoclast Function.Frontiers in cell and developmental biology · 2020Article
- Deficiency in the phosphatase PHLPP1 suppresses osteoclast-mediated bone resorption and enhances bone formation in mice.The Journal of biological chemistry · 2019Article
- mTORC1 impedes osteoclast differentiation via calcineurin and NFATc1.Communications biology · 2018Article
- Inhibition of osteoclast differentiation and collagen antibody-induced arthritis by CTHRC1.Bone · 2017Article
- Deletion of Rac in Mature Osteoclasts Causes Osteopetrosis, an Age-Dependent Change in Osteoclast Number, and a Reduced Number of Osteoblasts In Vivo.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2016Article
- Foxn1[Cre] Expression in the Male Germline.PloS one · 2016Article
- Tissue Specific Expression of Cre in Rat Tyrosine Hydroxylase and Dopamine Active Transporter-Positive Neurons.PloS one · 2016Article
- Ablation of Tak1 in osteoclast progenitor leads to defects in skeletal growth and bone remodeling in mice.Scientific reports · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Osteoclasts are terminally differentiated cells that attach to bone and secrete proteases to degrade the bone matrix. The primary protease responsible for the degradation of the organic component of the bone matrix is Cathepsin K, which was largely thought to be unique to osteoclasts. Given its apparent selective expression in osteoclasts, the Cathepsin K promoter has been engineered to drive the expression of Cre recombinase in mice and has been the most relevant tool for generating osteoclast-specific gene loss. In an effort to understand the role of the ARF tumor suppressor in osteoclasts, we crossed Arf (fl/fl) mice to Ctsk(Cre/+) mice, which unexpectedly resulted in the germline loss of Arf. We subsequently confirmed Cre activity in gametes by generating Ctsk(Cre/+); Rosa(+) mice. These results raise significant concerns regarding in vivo bone phenotypes created using Ctsk(Cre/+) mice and warrant further investigation into the role of Cathepsin K in gametes as well as alternative tools for studying osteoclast-specific gene loss in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.