ArticlePloS one2012
Erwinia amylovora CRISPR elements provide new tools for evaluating strain diversity and for microbial source tracking.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 96 citations in OpenAlex.
- Pathogenicity and virulence ofVirulence · 2026Review
- Development of strain specific simple sequence repeats and assessment of genetic diversity in Erwinia amylovora from marker selection to phylogenetic analysis.Scientific reports · 2025Article
- Genetic Diversity and Genotype Distribution of Erwinia amylovora in Korea.The plant pathology journal · 2025Article
- Unveiling Antibiotic Resistance: Genome Sequencing of Streptomycin-ResistantMicroorganisms · 2024Article
- Intraspecific diversity of Erwinia amylovora strains from northern Algeria.BMC microbiology · 2024Article
- Epidemiological description of fire blight introduction patterns to Central Asia and the Caucasus region based on CRISPR spacer typing and genome analysis.Phytopathology research · 2024Article
- Novel approach toward the understanding of genetic diversity based on the two types of amino acid repeats in Erwinia amylovora.Scientific reports · 2023Article
- A complete twelve-gene deletion null mutant reveals that cyclic di-GMP is a global regulator of phase-transition and host colonization in Erwinia amylovora.PLoS pathogens · 2022Article
- Function and Application of the CRISPR-Cas System in the Plant PathogenApplied and environmental microbiology · 2022Article
- Variability within a clonal population ofPeerJ · 2022Article
- Tools and challenges to exploit microbial communities in agriculture.Current research in microbial sciences · 2021Review
- Comprehensive Molecular Dissection ofInternational journal of molecular sciences · 2021Article
- CRISPR loci-PCR as Tool for TrackingMicroorganisms · 2021Article
- Article
- CRISPR genotyping as complementary tool for epidemiological surveillance of Erwinia amylovora outbreaks.PloS one · 2021Article
- Genomic and Phenotypic Biology of Novel Strains ofFrontiers in plant science · 2021Article
- Inhibition of Erwinia amylovora by Bacillus nakamurai.Current microbiology · 2020Article
- Effectiveness of Plant Beneficial Microbes: Overview of the Methodological Approaches for the Assessment of Root Colonization and Persistence.Frontiers in plant science · 2020Review
- CRISPR elements provide a new framework for the genealogy of the citrus canker pathogen Xanthomonas citri pv. citri.BMC genomics · 2019Article
- Diversity of CRISPR/Cas system in Clostridium perfringens.Molecular genetics and genomics : MGG · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clustered regularly interspaced short palindromic repeats (CRISPRs) comprise a family of short DNA repeat sequences that are separated by non repetitive spacer sequences and, in combination with a suite of Cas proteins, are thought to function as an adaptive immune system against invading DNA. The number of CRISPR arrays in a bacterial chromosome is variable, and the content of each array can differ in both repeat number and in the presence or absence of specific spacers. We utilized a comparative sequence analysis of CRISPR arrays of the plant pathogen Erwinia amylovora to uncover previously unknown genetic diversity in this species. A total of 85 E. amylovora strains varying in geographic isolation (North America, Europe, New Zealand, and the Middle East), host range, plasmid content, and streptomycin sensitivity/resistance were evaluated for CRISPR array number and spacer variability. From these strains, 588 unique spacers were identified in the three CRISPR arrays present in E. amylovora, and these arrays could be categorized into 20, 17, and 2 patterns types, respectively. Analysis of the relatedness of spacer content differentiated most apple and pear strains isolated in the eastern U.S. from western U.S. strains. In addition, we identified North American strains that shared CRISPR genotypes with strains isolated on other continents. E. amylovora strains from Rubus and Indian hawthorn contained mostly unique spacers compared to apple and pear strains, while strains from loquat shared 79% of spacers with apple and pear strains. Approximately 23% of the spacers matched known sequences, with 16% targeting plasmids and 5% targeting bacteriophage. The plasmid pEU30, isolated in E. amylovora strains from the western U.S., was targeted by 55 spacers. Lastly, we used spacer patterns and content to determine that streptomycin-resistant strains of E. amylovora from Michigan were low in diversity and matched corresponding streptomycin-sensitive strains from the background population.
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