ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2012
The rates of postmortem proteolysis of glutamate transporters differ dramatically between cells and between transporter subtypes.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Postmortem changes in brain cell structure: a review.Free neuropathology · 2023Article
- iGluR expression in the hippocampal formation, entorhinal cortex, and superior temporal gyrus in Alzheimer's disease.Neural regeneration research · 2022Article
- Reconstitution of GABA, Glycine and Glutamate Transporters.Neurochemical research · 2022Review
- Network-Related Changes in Neurotransmitters and Seizure Propagation During Rodent Epileptogenesis.Neurology · 2021Article
- Potassium and glutamate transport is impaired in scar-forming tumor-associated astrocytes.Neurochemistry international · 2020Article
- Astrocytes and Glutamine Synthetase in Epileptogenesis.Journal of neuroscience research · 2019Review
- Selective deletion of glutamine synthetase in the mouse cerebral cortex induces glial dysfunction and vascular impairment that precede epilepsy and neurodegeneration.Neurochemistry international · 2019Article
- Reactive astrogliosis causes the development of spontaneous seizures.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- EAAT2 (GLT-1; slc1a2) glutamate transporters reconstituted in liposomes argues against heteroexchange being substantially faster than net uptake.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Localization of excitatory amino acid transporters EAAT1 and EAAT2 in human postmortem cortex: a light and electron microscopic study.Neuroscience · 2014Article
- Glutamate as a neurotransmitter in the healthy brain.Journal of neural transmission (Vienna, Austria : 1996) · 2014Review
- Proteome analysis and conditional deletion of the EAAT2 glutamate transporter provide evidence against a role of EAAT2 in pancreatic insulin secretion in mice.The Journal of biological chemistry · 2014Article
- GABA and Glutamate Transporters in Brain.Frontiers in endocrinology · 2013Review
- Deletion of the γ-aminobutyric acid transporter 2 (GAT2 and SLC6A13) gene in mice leads to changes in liver and brain taurine contents.The Journal of biological chemistry · 2012Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glutamate transporters (GLT-1, GLAST, EAAC1) limit the actions of excitatory amino acids. Because a disturbed transporter operation can cause or aggravate neurological diseases, transporters are of considerable neuropathological interest. Human samples, however, are seldom obtained fresh. Here, we used mice brains to study how fast glutamate transporters are degraded after death. Immunoblots showed that terminal GLT-1 epitopes (within residues 1-26 and 518-573) had mostly disappeared after 24 hr. GLAST termini (1-25 and 522-543) degraded slightly slower. In contrast, epitopes within central parts of GLT-1 (493-508) and the EAAC1 C-terminus (510-523) were readily detectable after 72 hr. The decline in immunoreactivity of the GLT-1 and GLAST termini was also seen in tissue sections, but proteolysis did not happen synchronously in all cells. At 24 hr, scattered cells remained strongly immunopositive, while the majority of cells were completely immunonegative. GLAST and GLT-1 co-localized in neocortical tissue, but at 12 hr, many GLAST-positive cells had lost the GLT-1 termini. The uneven disappearance of labeling was not observed with the antibodies to GLT-1 residues 493-508. The immunoreactivity to this epitope correlated better with the reported glutamate uptake activity. Thus, postmortem delay may affect epitopes differently, possibly causing erroneous conclusions about relative expression levels.
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