ArticlePlant physiology2012
A chimeric arabinogalactan protein promotes somatic embryogenesis in cotton cell culture.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
- Genomic atlas of phytocyanin genes for improvement of climate resilience in bread wheat.BMC plant biology · 2026Article
- Secreted molecules as modulators of somatic embryogenesis efficiency - an overview.Frontiers in plant science · 2026Review
- Somatic Embryogenesis: A Biotechnological Route in the Production of Recombinant Proteins.Biotech (Basel (Switzerland)) · 2025Review
- Expression of a multigene mushroom luciferin biosynthesis pathway as a pseudo-polycistron in plants.Scientific reports · 2025Article
- Genome-wide identification unravels the role of the arabinogalactan peptide (AGP) gene family in cotton plant architecture.Plant cell reports · 2025Article
- Effect of Arabinogalactans on Induction of White-Opaque Somatic Embryos of Avocado (Plants (Basel, Switzerland) · 2023Article
- Distinct functions of FASCILIN-LIKE ARABINOGALACTAN PROTEINS relate to domain structure.Plant physiology · 2023Article
- miR171 modulates induction of somatic embryogenesis in citrus callus.Plant cell reports · 2022Article
- Weighted Gene Correlation Network Analysis (WGCNA) ofInternational journal of genomics · 2022Article
- Article
- On the Potential Function of Type II ArabinogalactanFrontiers in plant science · 2020Review
- Identification of Hydroxyproline-Containing Proteins and Hydroxylation of Proline Residues in Rice.Frontiers in plant science · 2020Article
- Genomic dissection of an extended phenotype: Oak galling by a cynipid gall wasp.PLoS genetics · 2019Article
- Metabolome and Transcriptome Association Analysis Reveals Dynamic Regulation of Purine Metabolism and Flavonoid Synthesis in Transdifferentiation during Somatic Embryogenesis in Cotton.International journal of molecular sciences · 2019Article
- Cell Wall Epitopes and Endoploidy as Reporters of Embryogenic Potential inInternational journal of molecular sciences · 2018Article
- Genome-wide identification, classification, and expression of phytocyanins in Populus trichocarpa.Planta · 2018Article
- Pectin De-methylesterification and AGP Increase Promote Cell Wall Remodeling and Are Required During Somatic Embryogenesis ofFrontiers in plant science · 2018Article
- Arabidopsis thaliana FLA4 functions as a glycan-stabilized soluble factor via its carboxy-proximal Fasciclin 1 domain.The Plant journal : for cell and molecular biology · 2017Article
- Nanospherical arabinogalactan proteins are a key component of the high-strength adhesive secreted by English ivy.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Label-Free Quantitative Proteomics of Embryogenic and Non-Embryogenic Callus during Sugarcane Somatic Embryogenesis.PloS one · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabinogalactan proteins (AGPs) are a family of extracellular plant proteoglycans implicated in many aspects of plant growth and development, including in vitro somatic embryogenesis (SE). We found that specific AGPs were produced by cotton (Gossypium hirsutum) calli undergoing SE and that when these AGPs were isolated and incorporated into tissue culture medium, cotton SE was promoted. When the AGPs were partly or fully deglycosylated, SE-promoting activity was not diminished. Testing of AGPs separated by reverse-phase high-performance liquid chromatography revealed that the SE-promoting activity resided in a hydrophobic fraction. We cloned a full-length complementary DNA (cotton PHYTOCYANIN-LIKE ARABINOGALACTAN-PROTEIN1 [GhPLA1]) that encoded the protein backbone of an AGP in the active fraction. It has a chimeric structure comprising an amino-terminal signal sequence, a phytocyanin-like domain, an AGP-like domain, and a hydrophobic carboxyl-terminal domain. Recombinant production of GhPLA1 in tobacco (Nicotiana tabacum) cells enabled us to purify and analyze a single glycosylated AGP and to demonstrate that this chimeric AGP promotes cotton SE. Furthermore, the nonglycosylated phytocyanin-like domain from GhPLA1, which was bacterially produced, also promoted SE, indicating that the glycosylated AGP domain was unnecessary for in vitro activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.