ArticleProstaglandins, leukotrienes, and essential fatty acids
Acute effects of prostaglandin E1 and E2 on vascular reactivity and blood flow in situ in the chick chorioallantoic membrane.
D R Harland, L D Lorenz, K Fay, B E Dunn, S K Gruenloh, J Narayanan, E R Jacobs, M Medhora
Abstract read
In one paragraphArticle in Prostaglandins, leukotrienes, and essential fatty acids. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
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3 · Its place in the literatureWho cites it
0 citing papers in PubMed, 4 citations in OpenAlex.
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4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
8 authors at 2 institutions in 1 country.
D R Harland *Division of Pulmonary and Critical Care, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226.
L D Lorenz *Division of Pulmonary and Critical Care, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226.
K FayDivision of Pulmonary and Critical Care, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226.
B E DunnDivision of Pulmonary and Critical Care, Department of Pathology, Medical College of Wisconsin, Milwaukee, WI 53226.
S K GruenlohDivision of Pulmonary and Critical Care, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226.
J NarayananDivision of Pulmonary and Critical Care, Department of Radiation Oncology, Medical College of Wisconsin, Milwaukee, WI 53226.
E R JacobsDivision of Pulmonary and Critical Care, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226.
M MedhoraDivision of Pulmonary and Critical Care, Department of Radiation Oncology, Medical College of Wisconsin, Milwaukee, WI 53226.
Medical College of Wisconsin · USMilwaukee VA Medical Center · US
Funding
PULMONARY ARTERY ENDOTHELIAL CELLS AND SHEAR STRESSR01HL049294 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI JACOBS, ELIZABETH R · 1994 to 2010
$3.1MMechanisms of High Flow Induced VasculopathyR01HL068627 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI JACOBS, ELIZABETH R. · 2003 to 2007
$1.6MRole of EETs in Growth of Human Endothelial CellsR01HL069996 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI MEDHORA, MEETHA M · 2003 to 2006
$1.3MNovel imaging to identify lung mitochondrial injury and predict recoveryR01HL116530 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI JACOBS, ELIZABETH R · 2013 to 2016
$1.1MNHLBI NIH HHS HL069996NHLBI NIH HHS HL49294NHLBI NIH HHS HL68627NHLBI NIH HHS R01 HL049294NHLBI NIH HHS R01 HL069996NHLBI NIH HHS R01 HL116530
6 · The paper itselfAbstract
The chick chorioallantoic membrane (CAM) subserves gas exchange in the developing embryo and shell-less culture affords a unique opportunity for direct observations over time of individual blood vessels to pharmacologic interventions. We tested a number of lipids including prostaglandins PGE(1&2) for vascular effects and signaling in the CAM. Application of PGE(1&2) induced a decrease in the diameter of large blood vessels and a concentration-dependent, localized, reversible loss of blood flow through small vessels. The loss of flow was also mimicked by misoprostol, an agonist for 3 of 4 known PGE receptors, EP(2-4), and by U46619, a thromboxane mimetic. Selective receptor antagonists for EP(3) and thromboxane each partially blocked the response. This is a first report of the effects of prostaglandins on vasoreactivity in the CAM. Our model allows the unique ability to examine simultaneous responses of large and small vessels in real time and in vivo.
Indexed as
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acidalpha-Linolenic AcidAlprostadilAnimalsBiphenyl CompoundsBlood VesselsBridged Bicyclo Compounds, HeterocyclicChick EmbryoChorioallantoic MembraneDibenz(b,f)(1,4)oxazepine-10(11H)-carboxylic acid, 8-chloro-, 2-acetylhydrazideDinoprostoneDose-Response Relationship, DrugEstradiolEstrogensFatty Acids, UnsaturatedHydrazines15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid6-isopropoxy-9-oxoxanthene-2-carboxylic acidAH 23848alpha-Linolenic AcidAlprostadilBiphenyl CompoundsBridged Bicyclo Compounds, HeterocyclicDibenz(b,f)(1,4)oxazepine-10(11H)-carboxylic acid, 8-chloro-, 2-acetylhydrazideDinoprostoneEstradiolEstrogensFatty Acids, UnsaturatedHydrazinesMisoprostolProstaglandin AntagonistsReceptors, ProstaglandinReceptors, ThromboxaneSQ 29548Vasoconstrictor AgentsXanthones
Identifiers
PMID22858445
PMCPMC3839254
OpenAlexW2070797616
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