ReviewMethods in cell biology2012
Picking faces out of a crowd: genetic labels for identification of proteins in correlated light and electron microscopy imaging.
Review in Methods in cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- Article
- The design of functional proteins using tensorized energy calculations.Cell reports methods · 2023Article
- Proteomic mapping and optogenetic manipulation of membrane contact sites.The Biochemical journal · 2022Article
- Unconventional tonicity-regulated nuclear trafficking of NFAT5 mediated by KPNB1, XPOT and RUVBL2.Journal of cell science · 2022Article
- Ultrastructure and nuclear architecture of telomeric chromatin revealed by correlative light and electron microscopy.Nucleic acids research · 2022Article
- Correlative Light-Environmental Scanning Electron Microscopy of Plasma Membrane Efflux Carriers of Plant Hormone Auxin.Biomolecules · 2021Article
- Targeting Functionally Characterized Synaptic Architecture Using Inherent Fiducials and 3D Correlative Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2021Article
- Proximity-dependent labeling methods for proteomic profiling in living cells: An update.Wiley interdisciplinary reviews. Developmental biology · 2021Review
- Assaying three-dimensional cellular architecture using X-ray tomographic and correlated imaging approaches.The Journal of biological chemistry · 2020Review
- Imaging VIPER-labeled Cellular Proteins by Correlative Light and Electron Microscopy.Bio-protocol · 2019Article
- Generation of CoilR Probe Peptides for VIPER-labeling of Cellular Proteins.Bio-protocol · 2019Article
- Imaging cell morphology and physiology using X-rays.Biochemical Society transactions · 2019Review
- VIPER is a genetically encoded peptide tag for fluorescence and electron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Blue-Light Receptors for Optogenetics.Chemical reviews · 2018Review
- Proximity-dependent labeling methods for proteomic profiling in living cells.Wiley interdisciplinary reviews. Developmental biology · 2017Review
- Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy.Journal of visualized experiments : JoVE · 2017Article
- Studying dyadic structure-function relationships: a review of current modeling approaches and new insights into CaClinical Medicine Insights. Cardiology · 2017Article
- Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell.Biology of the cell · 2017Review
- Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2.Nature protocols · 2016Article
- Vectors for Genetically-Encoded Tags for Electron Microscopy Contrast in Drosophila.Biological procedures online · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Correlated light and electron microscopic (CLEM) imaging is a powerful method for dissecting cell and tissue function at high resolution. Each imaging mode provides unique information, and the combination of the two can contribute to a better understanding of the spatiotemporal patterns of protein expression, trafficking, and function. Critical to these methods is the use of genetically appended tags that highlight specific proteins of interest in order to be able to pick them out of their complex cellular environment. Here we review and discuss the current generation of genetic labels for direct protein identification by CLEM, addressing their relative strengths and weaknesses and in what experiments they would be most useful.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.