ArticleBMC plant biology2012
A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus.
Article in BMC plant biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 112 citations in OpenAlex.
- Integrated transcriptomic and metabolomic analyses reveal phytohormone signaling mechanisms underlying differential regeneration potential in microspore-derived calli from hulled and hulless barley.BMC plant biology · 2026Article
- The effect of phytosulfokine alpha on haploid embryogenesis and gene expression ofFrontiers in plant science · 2024Article
- Transcriptomics integrated with metabolomics reveals the effect of cold stress on rice microspores.BMC plant biology · 2023Article
- Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore.International journal of molecular sciences · 2023Article
- Review
- Dynamics of Endogenous Auxin and Its Role in Somatic Embryogenesis Induction and Progression in Cork Oak.Plants (Basel, Switzerland) · 2023Article
- The Roads to Haploid Embryogenesis.Plants (Basel, Switzerland) · 2023Review
- Anther Culture-Derived Haploids ofPlants (Basel, Switzerland) · 2022Article
- Cellular responses of oil palm genotypes during somatic embryogenesis involve participation of procambial cells, DNA demethylation, and auxin accumulation.Plant cell reports · 2022Article
- Article
- Small molecule inhibitors of mammalian GSK-3β promote in vitro plant cell reprogramming and somatic embryogenesis in crop and forest species.Journal of experimental botany · 2021Article
- Embryogenesis of European Radish (Plants (Basel, Switzerland) · 2021Article
- Understanding In Vitro Tissue Culture-Induced Variation Phenomenon in Microspore System.International journal of molecular sciences · 2021Review
- Suppression of Metacaspase- and Autophagy-Dependent Cell Death Improves Stress-Induced Microspore Embryogenesis in Brassica napus.Plant & cell physiology · 2021Article
- Species with Haploid or Doubled Haploid Protocols.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The Resistance of Oilseed Rape Microspore-Derived Embryos to Osmotic Stress Is Associated With the Accumulation of Energy Metabolism Proteins, Redox Homeostasis, Higher Abscisic Acid, and Cytokinin Contents.Frontiers in plant science · 2021Article
- Live Imaging of embryogenic structures in Brassica napus microspore embryo cultures highlights the developmental plasticity of induced totipotent cells.Plant reproduction · 2020Article
- Advances in Plant Regeneration: Shake, Rattle and Roll.Plants (Basel, Switzerland) · 2020Review
- In vitro androgenesis: spontaneous vs. artificial genome doubling and characterization of regenerants.Plant cell reports · 2020Review
- Identification, Gene Structure, and Expression ofFrontiers in plant science · 2020Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicrospore embryogenesis represents a unique system of single cell reprogramming in plants wherein a highly specialized cell, the microspore, by specific stress treatment, switches its fate towards an embryogenesis pathway. In Brassica napus, a model species for this phenomenon, incubation of isolated microspores at 32°C is considered to be a pre-requisite for embryogenesis induction.
resultsWe have developed a new in vitro system at lower temperature (18°C) to efficiently induce microspore embryogenesis throughout two different developmental pathways: one involving the formation of suspensor-like structures (52.4%) and another producing multicellular embryos without suspensor (13.1%); additionally, a small proportion of non-responsive microspores followed a gametophytic-like development (34.4%) leading to mature pollen. The suspensor-like pathway followed at 18°C involved the establishment of asymmetric identities from the first microspore division and an early polarity leading to different cell fates, suspensor and embryo development, which were formed by cells with different organizations and endogenous auxin distribution, similar to zygotic embryogenesis. In addition, a new strategy for germination of microspore derived embryos was developed for achieving more than 90% conversion of embryos to plantlets, with a predominance of spontaneous doubled haploids plants.
conclusionThe present work reveals a novel mechanism for efficient microspore embryogenesis induction in B. napus using continuous low temperature treatment. Results indicated that low temperature applied for longer periods favours an embryogenesis pathway whose first division originates asymmetric cell identities, early polarity establishment and the formation of suspensor-like structures, mimicking zygotic embryogenesis. This new in vitro system provides a convenient tool to analyze in situ the mechanisms underlying different developmental pathways during the microspore reprogramming, breaking or not the cellular symmetry, the establishment of polarity and the developmental embryo patterning, which further produce mature embryos and plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.