ArticleMolecular biology of the cell2012
Actin polymerization controls the activation of multidrug efflux at fertilization by translocation and fine-scale positioning of ABCB1 on microvilli.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- Myosin Vb Traffics P-Glycoprotein to the Apical Membrane of Intestinal Epithelial Cells.Gastroenterology · 2025Article
- Human Sperm Chromosomes: To Form Hairpin-Loops, Or Not to Form Hairpin-Loops, That Is the Question.Genes · 2019Article
- A new model of sperm nuclear architecture following assessment of the organization of centromeres and telomeres in three-dimensions.Scientific reports · 2017Article
- Endosome-mitochondria interactions are modulated by iron release from transferrin.The Journal of cell biology · 2016Article
- Functional diversification of sea urchin ABCC1 (MRP1) by alternative splicing.American journal of physiology. Cell physiology · 2016Article
- Focusing super resolution on the cytoskeleton.F1000Research · 2016Review
- ABCC5 is required for cAMP-mediated hindgut invagination in sea urchin embryos.Development (Cambridge, England) · 2015Article
- In vivo imaging of Nematostella vectensis embryogenesis and late development using fluorescent probes.BMC cell biology · 2014Article
- Transport in technicolor: mapping ATP-binding cassette transporters in sea urchin embryos.Molecular reproduction and development · 2014Review
- Integrins on eggs: focal adhesion kinase is activated at fertilization, forms a complex with integrins, and is necessary for cortex formation and cell cycle initiation.Molecular biology of the cell · 2013Article
- Localization and substrate selectivity of sea urchin multidrug (MDR) efflux transporters.The Journal of biological chemistry · 2012Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Fertilization changes the structure and function of the cell surface. In sea urchins, these changes include polymerization of cortical actin and a coincident, switch-like increase in the activity of the multidrug efflux transporter ABCB1a. However, it is not clear how cortical reorganization leads to changes in membrane transport physiology. In this study, we used three-dimensional superresolution fluorescence microscopy to resolve the fine-scale movements of the transporter along polymerizing actin filaments, and we show that efflux activity is established after ABCB1a translocates to the tips of the microvilli. Inhibition of actin polymerization or bundle formation prevents tip localization, resulting in the patching of ABCB1a at the cell surface and decreased efflux activity. In contrast, enhanced actin polymerization promotes tip localization. Finally, interference with Rab11, a regulator of apical recycling, inhibits activation of efflux activity in embryos. Together our results show that actin-mediated, short-range traffic and positioning of transporters at the cell surface regulates multidrug efflux activity and highlight the multifaceted roles of microvilli in the spatial distribution of membrane proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.