ArticleMolecular biology of the cell2012
A nuclear-derived proteinaceous matrix embeds the microtubule spindle apparatus during mitosis.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Mitotic spindle membranes.Molecular biology of the cell · 2025Review
- Roles of Tubulin Concentration during Prometaphase and Ran-GTP during Anaphase ofLife science alliance · 2024Article
- Roles of Tubulin Concentration during Prometaphase and Ran-GTP during Anaphase ofbioRxiv : the preprint server for biology · 2024Article
- Sequential accumulation of dynein and its regulatory proteins at the spindle region in the Caenorhabditis elegans embryo.Scientific reports · 2022Article
- Review
- Spatiotemporal coordination of Greatwall-Endos-PP2A promotes mitotic progression.The Journal of cell biology · 2021Article
- Nuclear envelope remodelling during mitosis.Current opinion in cell biology · 2021Review
- Protein phase separation in mitosis.Current opinion in cell biology · 2019Review
- Precocious cleavage furrows simultaneously move and ingress when kinetochore microtubules are depolymerized in Mesostoma ehrenbergii spermatocytes.Protoplasma · 2018Article
- Article
- Phase separation of BuGZ promotes Aurora A activation and spindle assembly.The Journal of cell biology · 2018Article
- Evidence for a role of spindle matrix formation in cell cycle progression by antibody perturbation.PloS one · 2018Article
- An intrinsic mechanism controls reactivation of neural stem cells by spindle matrix proteins.Nature communications · 2017Article
- "Wait anaphase" signals are not confined to the mitotic spindle.Molecular biology of the cell · 2017Article
- Digitor/dASCIZ Has Multiple Roles in Drosophila Development.PloS one · 2016Article
- Article
- An organelle-exclusion envelope assists mitosis and underlies distinct molecular crowding in the spindle region.The Journal of cell biology · 2015Article
- Article
- Movement of chromosomes with severed kinetochore microtubules.Protoplasma · 2015Article
- A computational model for the formation of lamin-B mitotic spindle envelope and matrix.Interface focus · 2014Article
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Authors and funding
7 authors.
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Abstract
The concept of a spindle matrix has long been proposed. Whether such a structure exists, however, and what its molecular and structural composition are have remained controversial. In this study, using a live-imaging approach in Drosophila syncytial embryos, we demonstrate that nuclear proteins reorganize during mitosis to form a highly dynamic, viscous spindle matrix that embeds the microtubule spindle apparatus, stretching from pole to pole. We show that this "internal" matrix is a distinct structure from the microtubule spindle and from a lamin B-containing spindle envelope. By injection of 2000-kDa dextran, we show that the disassembling nuclear envelope does not present a diffusion barrier. Furthermore, when microtubules are depolymerized with colchicine just before metaphase the spindle matrix contracts and coalesces around the chromosomes, suggesting that microtubules act as "struts" stretching the spindle matrix. In addition, we demonstrate that the spindle matrix protein Megator requires its coiled-coil amino-terminal domain for spindle matrix localization, suggesting that specific interactions between spindle matrix molecules are necessary for them to form a complex confined to the spindle region. The demonstration of an embedding spindle matrix lays the groundwork for a more complete understanding of microtubule dynamics and of the viscoelastic properties of the spindle during cell division.
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