ArticleMarine biotechnology (New York, N.Y.)2013
Isolation and characterization of male-specific DNA markers in the rock bream Oplegnathus fasciatus.
Article in Marine biotechnology (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- Understanding Genetic Regulation of Sex Differentiation in Hermaphroditic Fish.Animals : an open access journal from MDPI · 2025Review
- Advances in biotechnology and breeding innovations in China's marine aquaculture.Advanced biotechnology · 2024Review
- Innovative approach for high-throughput exploiting sex-specific markers in Japanese parrotfish Oplegnathus fasciatus.GigaScience · 2024Article
- Article
- Sex-Specific Genomic Region Identification and Molecular Sex Marker Development of Rock Bream (Oplegnathus fasciatus).Marine biotechnology (New York, N.Y.) · 2022Article
- Exploration of an XX/XY Sex Determination System and Development of PCR-Based Sex-specific Markers inFrontiers in genetics · 2022Article
- A simple and rapid method for fish sex identification based on recombinase-aided amplification and its use in Cynoglossus semilaevis.Scientific reports · 2021Article
- Identification and Characterization of Dimorphic Expression of Sex-Related Genes in Rock Bream, a Fish With Multiple Sex Chromosomes.Frontiers in genetics · 2021Article
- Isolation of a Male-Specific Molecular Marker and Development of a Genetic Sex Identification Technique in Spotted Knifejaw (Oplegnathus punctatus).Marine biotechnology (New York, N.Y.) · 2020Article
- Transcriptomic analyses reveal novel genes with sexually dimorphic expression in Takifugu rubripes brain during gonadal sex differentiation.Genes & genomics · 2020Article
- Deciphering the Origin and Evolution of the XInternational journal of molecular sciences · 2019Article
- Article
- Discordant population histories of host and its parasite: A role for ecological permeability of extreme environment?PloS one · 2017Article
- The testis and ovary transcriptomes of the rock bream (Oplegnathus fasciatus): A bony fish with a unique neo Y chromosome.Genomics data · 2016Article
- Transcriptomic Analyses Reveal Novel Genes with Sexually Dimorphic Expression in Yellow Catfish (Pelteobagrus fulvidraco) Brain.Marine biotechnology (New York, N.Y.) · 2015Article
- Identification of Sex-Linked SNPs and Sex-Determining Regions in the Yellowtail Genome.Marine biotechnology (New York, N.Y.) · 2015Article
- Identification of Sex-Specific Markers Reveals Male Heterogametic Sex Determination in Pseudobagrus ussuriensis.Marine biotechnology (New York, N.Y.) · 2015Article
- Chromosomal mapping of microsatellite repeats in the rock bream fish Oplegnathus fasciatus, with emphasis of their distribution in the neo-Y chromosome.Molecular cytogenetics · 2013Article
- Genetic differentiation and efficient sex-specific marker development of a pair of Y- and X-linked markers in yellow catfish.International journal of biological sciences · 2013Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sex-specific DNA markers applicable were very useful for elucidating the sex-determination mechanism and sex control in fishes. In the present study, amplified fragment-length polymorphism (AFLP) approach with 144 primer combinations was employed to identify sex-specific markers in the rock bream. Four male-specific AFLP fragments were identified which were designated as Opl286, Opl237, Opl422, and Opl228. Further sequence analysis of the sex markers' genomic region revealed subtle differences between the males and females. We identified four male-specific single-nucleotide polymorphisms (SNPs) and a deletion of 8 bp in marker Opl286, six male-specific SNPs in marker Opl237, three male-specific SNPs in marker Opl422, and eight male-specific SNPs and 1 bp inversions in marker Opl228. Specific primers were designed based on the nucleotide variation in the sequences to develop a simple polymerase chain reaction method for identifying the genetic sex of rock bream. As a result, three out of the four male-specific markers were converted into SNP markers. The male-specific AFLP markers and AFLP-derived SNP markers were tested in 100 individuals collected from three locations around the coast of Zhoushan, yielding reproducible sex identification. These male-specific DNA markers are a useful tool for the identification of the sex-determining locus in rock bream and for guiding artificial breeding programs.
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