ArticlePediatric nephrology (Berlin, Germany)2012
DNA methylation changes between relapse and remission of minimal change nephrotic syndrome.
Article in Pediatric nephrology (Berlin, Germany), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Epigenetic Mechanisms and Nephrotic Syndrome: A Systematic Review.Biomedicines · 2023Review
- Identifying the Potential Roles of PBX4 in Human Cancers Based on Integrative Analysis.Biomolecules · 2022Article
- An evaluation of kidney dysfunction as a common symptom of typhoid infection in an endemic country: A rare case study.IDCases · 2022Article
- Trends in pediatric nephrotic syndrome.World journal of nephrology · 2021Review
- Screening and Analysis of Key Genes in miRNA-mRNA Regulatory Network of Membranous Nephropathy.Journal of healthcare engineering · 2021Article
- Ascorbic acid during the suckling period is required for proper DNA demethylation in the liver.Scientific reports · 2020Article
- Epigenetic modulation of Fgf21 in the perinatal mouse liver ameliorates diet-induced obesity in adulthood.Nature communications · 2018Article
- Changes in DNA methylation in naïve T helper cells regulate the pathophysiological state in minimal-change nephrotic syndrome.BMC research notes · 2017Article
- Cell biology and genetics of minimal change disease.F1000Research · 2016Review
- B-RAF mutation and accumulated gene methylation in aberrant crypt foci (ACF), sessile serrated adenoma/polyp (SSA/P) and cancer in SSA/P.British journal of cancer · 2015Article
- Comparison of methylprednisolone plus prednisolone with prednisolone alone as initial treatment in adult-onset minimal change disease: a retrospective cohort study.Clinical journal of the American Society of Nephrology : CJASN · 2014Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDNA methylation of gene promoters is associated with transcriptional inactivation. Changes in DNA methylation can lead to differences in gene expression levels and thereby influence disease development. We hypothesized that epigenetics underlies the pathogenesis of minimal change nephrotic syndrome (MCNS).
methodsGenome-wide DNA methylation changes between relapse and remission in monocytes (n = 6) and naive T helper cells (Th0s) (n = 4) isolated from patients with MCNS were investigated using the microarray-based integrated analysis of methylation by isochizomers (MIAMI) method. We confirmed the MIAMI results using bisulfite-pyrosequencing analysis. Expression analysis was performed using quantitative real-time PCR.
resultsThree gene loci (GATA2, PBX4, and NYX) were significantly less methylated in Th0s during relapse than in remission, compared to none in monocytes. In addition, the distance distribution from the regression line of all probes in MIAMI was significantly different between monocytes and Th0s. The mRNA levels of the three genes in Th0s were not significantly different between relapse and remission.
conclusionsOur results demonstrate that the change in DNA methylation patterns from remission to relapse in MCNS occurs predominantly in Th0s rather than in monocytes and suggest that epigenetic regulation in Th0s underlies the pathogenesis of MCNS.
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