ArticleClinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico2012
The SGBS cell strain as a model for the in vitro study of obesity and cancer.
Article in Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 36 citations in OpenAlex.
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- Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models.Scientific data · 2023Article
- Stages of preadipocyte differentiation: biomarkers and pathways for extracellular structural remodeling.Hereditas · 2022Article
- Obesity III: Obesogen assays: Limitations, strengths, and new directions.Biochemical pharmacology · 2022Review
- Secretome of Adipose Tissue as the Key to Understanding the Endocrine Function of Adipose Tissue.International journal of molecular sciences · 2022Review
- Fractionation of Extracts from Black Chokeberry, Cranberry, and Pomegranate to Identify Compounds That Influence Lipid Metabolism.Foods (Basel, Switzerland) · 2022Article
- Coffee BioactiveBiomolecules · 2021Article
- Impaired mRNA splicing and proteostasis in preadipocytes in obesity-related metabolic disease.eLife · 2021Article
- Obesity-associated leptin promotes chemoresistance in colorectal cancer through YAP-dependent AXL upregulation.American journal of cancer research · 2021Article
- A new human adipocyte model with PTEN haploinsufficiency.Adipocyte · 2020Article
- DNA methylation during human adipogenesis and the impact of fructose.Genes & nutrition · 2020Article
- An in vitro model for hypertrophic adipocytes: Time-dependent adipocyte proteome and secretome changes under high glucose and high insulin conditions.Journal of cellular and molecular medicine · 2020Article
- A human preadipocyte cell strain with multipotent differentiation capability as an in vitro model for adipogenesis.In vitro cellular & developmental biology. Animal · 2020Article
- Glucose Restriction Plus Refeeding in Vitro Induce Changes of the Human Adipocyte Secretome with an Impact on Complement Factors and Cathepsins.International journal of molecular sciences · 2019Article
- Differentiating SGBS adipocytes respond to PPARγ stimulation, irisin and BMP7 by functional browning and beige characteristics.Scientific reports · 2019Article
- Antioxidant and Anti-Inflammatory Properties ofAntioxidants (Basel, Switzerland) · 2019Article
- Simpson-Golabi-Behmel syndrome human adipocytes reveal a changing phenotype throughout differentiation.Histochemistry and cell biology · 2018Article
- The therapeutic properties of resminostat for hepatocellular carcinoma.Oncoscience · 2018Article
- SGBS cells as a model of human adipocyte browning: A comprehensive comparative study with primary human white subcutaneous adipocytes.Scientific reports · 2017Article
- Systems view of adipogenesis via novel omics-driven and tissue-specific activity scoring of network functional modules.Scientific reports · 2016Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe murine adipocyte cell line 3T3-L1 is well characterised and used widely, while the human pre-adipocyte cell strain, Simpson-Golabi-Behmel Syndrome (SGBS), requires validation for use in human studies. Obesity is currently estimated to account for up to 41 % of the worldwide cancer burden. A human in vitro model system is required to elucidate the molecular mechanisms for this poorly understood association. This work investigates the relevance of the SGBS cell strain for obesity and cancer research in humans. MATERIALS AND
methodsPre-adipocyte 3T3-L1 and SGBS were differentiated according to standard protocols. Morphology was assessed by Oil Red O staining. Adipocyte-specific gene expression was measured by qPCR and biochemical function was assessed by glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity. Differential gene expression in oesophageal adenocarcinoma cell line OE33 following co-culture with SGBS or primary omental human adipocytes was investigated using Human Cancer Profiler qPCR arrays.
resultsDuring the process of differentiation, SGBS expressed higher levels of adipocyte-specific transcripts and fully differentiated SGBS expressed more similar morphology, transcript levels and biochemical function to primary omental adipocytes, relative to 3T3-L1. Co-culture with SGBS or primary omental adipocytes induced differential expression of genes involved in adhesion (ITGB3), angiogenesis (IGF1, TEK, TNF, VEGFA), apoptosis (GZMA, TERT) and invasion and metastasis (MMP9, TIMP3) in OE33 tumour cells.
conclusionsComparable adipocyte-specific gene expression, biochemical function and a shared induced gene signature in co-cultured OE33 cells indicate that SGBS is a relevant in vitro model for obesity and cancer research in humans.
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