ArticleAnnals of biomedical engineering2013
Microfluidic system for facilitated quantification of nanoparticle accumulation to cells under laminar flow.
Article in Annals of biomedical engineering, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Microfluidics for nano-drug delivery systems: From fundamentals to industrialization.Acta pharmaceutica Sinica. B · 2023Review
- Microfluidic T Cell Selection by Cellular Avidity.Advanced healthcare materials · 2022Article
- Multi-oscillation microrheologyLab on a chip · 2021Article
- Integrating Biophysics in Toxicology.Cells · 2020Review
- Evaluating Nanoparticles in Preclinical Research Using Microfluidic Systems.Micromachines · 2019Review
- Recent advances of controlled drug delivery using microfluidic platforms.Advanced drug delivery reviews · 2018Review
- Targeting cell adhesion molecules with nanoparticles using in vivo and flow-based in vitro models of atherosclerosis.Experimental biology and medicine (Maywood, N.J.) · 2017Review
- Targeting and therapeutic peptides in nanomedicine for atherosclerosis.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- Microfluidics-based 3D cell culture models: Utility in novel drug discovery and delivery research.Bioengineering & translational medicine · 2016Review
- Priming nanoparticle-guided diagnostics and therapeutics towards human organs-on-chips microphysiological system.Nano convergence · 2016Review
- The role of biological fluid and dynamic flow in the behavior and cellular interactions of gold nanoparticles.Journal of nanobiotechnology · 2015Article
- Synthetic tumor networks for screening drug delivery systems.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- In vitro investigation of silica nanoparticle uptake into human endothelial cells under physiological cyclic stretch.Particle and fibre toxicology · 2014Article
- Organ-on-a-chip platforms for studying drug delivery systems.Journal of controlled release : official journal of the Controlled Release Society · 2014Review
- Vascular targeting of nanocarriers: perplexing aspects of the seemingly straightforward paradigm.ACS nano · 2014Review
- Quantitation of nanoparticle accumulation in flow using optimized microfluidic chambers.Journal of drug targeting · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
The identification of novel, synthetic targeting ligands to endothelial receptors has led to the rapid development of targeted nanoparticles for drug, gene and imaging probe delivery. Central to development and optimization are effective models for assessing particle binding in vitro. Here, we developed a simple and cost effective method to quantitatively assess nanoparticle accumulation under physiologically-relevant laminar flow. We designed reversibly vacuum-sealed PDMS microfluidic chambers compatible with 35 mm petri dishes, which deliver uniform or gradient shear stress. These chambers have sufficient surface area for facile cell collection for particle accumulation quantitation through FACS. We tested this model by synthesizing and flowing liposomes coated with APN (K (D) ~ 300 μM) and VCAM-1-targeting (K (D) ~ 30 μM) peptides over HUVEC. Particle binding significantly increased with ligand concentration (up to 6 mol%) and decreased with excess PEG. While the accumulation of particles with the lower affinity ligand decreased with shear, accumulation of those with the higher affinity ligand was highest in a low shear environment (2.4 dyne/cm(2)), as compared with greater shear or the absence of shear. We describe here a robust flow chamber model that is applied to optimize the properties of 100 nm liposomes targeted to inflamed endothelium.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.