ArticleJournal of cell science2012
Real-time hyperspectral fluorescence imaging of pancreatic β-cell dynamics with the image mapping spectrometer.
Article in Journal of cell science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 61 citations in OpenAlex.
- Compact and ultracompact spectral imagers: technology and applications in biomedical imaging.Journal of biomedical optics · 2023Review
- Super-multiplexing excitation spectral microscopy with multiple fluorescence bands.Biomedical optics express · 2022Article
- Excitation spectral microscopy for highly multiplexed fluorescence imaging and quantitative biosensing.Light, science & applications · 2021Article
- Review
- Hyperspectral imaging and spectral unmixing for improving whole-body fluorescence cryo-imaging.Biomedical optics express · 2021Article
- Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Full-field spectral-domain optical interferometry for snapshot three-dimensional microscopy.Biomedical optics express · 2020Article
- Development of a fast calibration method for image mapping spectrometry.Applied optics · 2020Article
- PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells.Biophysical journal · 2020Article
- PGRMC1 effects on metabolism, genomic mutation and CpG methylation imply crucial roles in animal biology and disease.BMC molecular and cell biology · 2020Article
- Snapshot hyperspectral light field imaging using image mapping spectrometry.Optics letters · 2020Article
- A Dye-Free Analog to Retinal Angiography Using Hyperspectral Unmixing to Retrieve Oxyhemoglobin Abundance.Translational vision science & technology · 2019Article
- Fibre-based spectral ratio endomicroscopy for contrast enhancement of bacterial imaging and pulmonary autofluorescence.Biomedical optics express · 2019Article
- High performance image mapping spectrometer (IMS) for snapshot hyperspectral imaging applications.Optics express · 2019Article
- Hyperspectral imaging in highly scattering media by the spectral phasor approach using two filters.Biomedical optics express · 2018Article
- Carotenoid dynamics and lipid droplet containing astaxanthin in response to light in the green alga Haematococcus pluvialis.Scientific reports · 2018Article
- Autofluorescence hyperspectral imaging of radiofrequency ablation lesions in porcine cardiac tissue.Journal of biophotonics · 2017Article
- Snapshot hyperspectral retinal imaging using compact spectral resolving detector array.Journal of biophotonics · 2017Article
- Fluorescence hyperspectral imaging (fHSI) using a spectrally resolved detector array.Journal of biophotonics · 2017Article
- Development of a multimodal foveated endomicroscope for the detection of oral cancer.Biomedical optics express · 2017Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The development of multi-colored fluorescent proteins, nanocrystals and organic fluorophores, along with the resulting engineered biosensors, has revolutionized the study of protein localization and dynamics in living cells. Hyperspectral imaging has proven to be a useful approach for such studies, but this technique is often limited by low signal and insufficient temporal resolution. Here, we present an implementation of a snapshot hyperspectral imaging device, the image mapping spectrometer (IMS), which acquires full spectral information simultaneously from each pixel in the field without scanning. The IMS is capable of real-time signal capture from multiple fluorophores with high collection efficiency (∼65%) and image acquisition rate (up to 7.2 fps). To demonstrate the capabilities of the IMS in cellular applications, we have combined fluorescent protein (FP)-FRET and [Ca(2+)](i) biosensors to measure simultaneously intracellular cAMP and [Ca(2+)](i) signaling in pancreatic β-cells. Additionally, we have compared quantitatively the IMS detection efficiency with a laser-scanning confocal microscope.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.