ArticleJournal of cell science2012
Polo-like kinase is required for synaptonemal complex disassembly and phosphorylation in mouse spermatocytes.
Article in Journal of cell science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 103 citations in OpenAlex.
- Article
- Orchestrating homolog segregation in meiosis I: molecular logic and regulatory networks with emphasis on male metaphase I.Cell communication and signaling : CCS · 2026Review
- Article
- Testis-specific serine/threonine kinase 3 regulates the size of sperm reservoir in Anopheles stephensi.Molecular genetics and genomics : MGG · 2025Article
- UBE2J2 is essential for the progression of meiosis prophase I during spermatogenesis in mice.iScience · 2025Article
- Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.Scientific reports · 2024Article
- Spermatocytes have the capacity to segregate chromosomes despite centriole duplication failure.EMBO reports · 2024Article
- The Organotypic Culture of Mouse Seminiferous Tubules as a Reliable Methodology for the Study of Meiosis In Vitro.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Phosphoproteome analysis of the crosstalk between sumoylation and phosphorylation in mouse spermatocytes.Biochemical and biophysical research communications · 2023Article
- Broad phosphorylation mediated by testis-specific serine/threonine kinases contributes to spermiogenesis and male fertility.Nature communications · 2023Article
- Single-cell transcriptome analysis and in vitro differentiation of testicular cells reveal novel insights into male sterility of the interspecific hybrid cattle-yak.BMC genomics · 2023Article
- GRAS-1 is a novel regulator of early meiotic chromosome dynamics in C. elegans.PLoS genetics · 2023Article
- Structural maturation of SYCP1-mediated meiotic chromosome synapsis by SYCE3.Nature structural & molecular biology · 2023Article
- Recruitment of Polo-like kinase couples synapsis to meiotic progression via inactivation of CHK-2.eLife · 2023Article
- Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- PBAF chromatin remodeler complexes that mediate meiotic transitions in mouse.Development (Cambridge, England) · 2022Article
- Global phosphoproteomic analysis identified key kinases regulating male meiosis in mouse.Cellular and molecular life sciences : CMLS · 2022Article
- FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination.Clinical and translational medicine · 2022Article
- Understanding the Underlying Molecular Mechanisms of Meiotic Arrest during In Vitro Spermatogenesis in Rat Prepubertal Testicular Tissue.International journal of molecular sciences · 2022Article
- PLK1 depletion alters homologous recombination and synaptonemal complex disassembly events during mammalian spermatogenesis.Molecular biology of the cell · 2022Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
During meiosis, accurate coordination of the completion of homologous recombination and synaptonemal complex (SC) disassembly during the prophase to metaphase I (G2/MI) transition is essential to avoid aneuploid gametes and infertility. Previous studies have shown that kinase activity is required to promote meiotic prophase exit. The first step of the G2/MI transition is the disassembly of the central element components of the SC; however, the kinase(s) required to trigger this process remains unknown. Here we assess roles of polo-like kinases (PLKs) in mouse spermatocytes, both in vivo and during prophase exit induced ex vivo by the phosphatase inhibitor okadaic acid. All four PLKs are expressed during the first wave of spermatogenesis. Only PLK1 (not PLK2-4) localizes to the SC during the G2/MI transition. The SC central element proteins SYCP1, TEX12 and SYCE1 are phosphorylated during the G2/MI transition. However, treatment of pachytene spermatocytes with the PLK inhibitor BI 2536 prevented the okadaic-acid-induced meiotic prophase exit and inhibited phosphorylation of the central element proteins as well as their removal from the SC. Phosphorylation assays in vitro demonstrated that PLK1, but not PLK2-4, phosphorylates central element proteins SYCP1 and TEX12. These findings provide mechanistic details of the first stage of SC disassembly in mammalian spermatocytes, and reveal that PLK-mediated phosphorylation of central element proteins is required for meiotic prophase exit.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.