ArticleBiophysical journal2012
Spontaneous formation of two-dimensional and three-dimensional cholesterol crystals in single hydrated lipid bilayers.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Cholesterol Crystallization Tests the Limits of Microscopic Reversibility for Molecular Pathways of Growth and Dissolution.ACS central science · 2026Article
- Direct observation of cholesterol monohydrate crystallization.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Lipid Droplet Surface Promotes 3D Morphological Evolution of Non-Rhomboidal Cholesterol Crystals.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains.Membranes · 2023Review
- Rafting on the Plasma Membrane: Lipid Rafts in Signaling and Disease.Advances in experimental medicine and biology · 2023Review
- Polymorphism, Structure, and Nucleation of Cholesterol·HJournal of the American Chemical Society · 2022Article
- Apolipoprotein E Binding Drives Structural and Compositional Rearrangement of mRNA-Containing Lipid Nanoparticles.ACS nano · 2021Article
- Ultramorphological analysis of plaque advancement and cholesterol crystal formation in Ldlr knockout mouse atherosclerosis.Atherosclerosis · 2019Article
- Why Is Very High Cholesterol Content Beneficial for the Eye Lens but Negative for Other Organs?Nutrients · 2019Review
- Two polymorphic cholesterol monohydrate crystal structures form in macrophage culture models of atherosclerosis.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Macrophages Shed Excess Cholesterol in Unique Extracellular Structures Containing Cholesterol Microdomains.Arteriosclerosis, thrombosis, and vascular biology · 2018Article
- Development of Correlative Cryo-soft X-ray Tomography and Stochastic Reconstruction Microscopy. A Study of Cholesterol Crystal Early Formation in Cells.Journal of the American Chemical Society · 2016Article
- ABCA1 contributes to macrophage deposition of extracellular cholesterol.Journal of lipid research · 2015Article
- Neutron Scattering at the Intersection of Heart Health Science and Biophysics.Journal of cardiovascular development and disease · 2015Review
- The interaction between amyloid-β peptides and anionic lipid membranes containing cholesterol and melatonin.PloS one · 2014Article
- ABCG1-mediated generation of extracellular cholesterol microdomains.Journal of lipid research · 2014Article
- Accumulation of ordered ceramide-cholesterol domains in farber disease fibroblasts.JIMD reports · 2014Article
- Formation of cholesterol bilayer domains precedes formation of cholesterol crystals in cholesterol/dimyristoylphosphatidylcholine membranes: EPR and DSC studies.The journal of physical chemistry. B · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Grazing incidence x-ray diffraction measurements were performed on single hydrated bilayers and monolayers of Ceramide/Cholesterol/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocyholine at varying concentrations. There are substantial differences in the phase and structure behavior of the crystalline domains formed within the bilayers relative to the corresponding monolayers, due to interactions between the opposing lipid leaflets. Depending on the lipid composition, these interactions lead to phase separation and formation of cholesterol crystals. The cholesterol and ceramide/cholesterol mixed phases were further characterized at 37°C by immunolabeling with specific antibodies recognizing ordered molecular arrays of cholesterol. Previous studies have shown that cholesterol may nucleate in artificial membranes to form thick two-dimensional bilayer crystals. The study herein demonstrates further growth of cholesterol into three-dimensional crystals. We believe that these results may provide further insight into the formation of cholesterol crystals in early stages of atherosclerosis inflammation.
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