ArticleBiophysical journal2012
Properties of intermediate filament networks assembled from keratin 8 and 18 in the presence of Mg²+.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 41 citations in OpenAlex.
- Protein-based materials: Applications, modification and molecular design.Biodesign research · 2025Review
- Multiscale architecture: Mechanics of composite cytoskeletal networks.Biophysics reviews · 2022Review
- From Strain Stiffening to Softening-Rheological Characterization of Keratins 8 and 18 Networks Crosslinked via Electron Irradiation.Polymers · 2022Article
- Review
- Nonlinear Elastic and Inelastic Properties of Cells.Journal of biomechanical engineering · 2020Review
- Microbial Keratinase: Next Generation Green Catalyst and Prospective Applications.Frontiers in microbiology · 2020Review
- Case-control study of mineral concentrations of hoof horn tissue derived from feedlot cattle with toe tip necrosis syndrome (toe necrosis).The Canadian veterinary journal = La revue veterinaire canadienne · 2018Article
- Intermediate Filaments and the Plasma Membrane.Cold Spring Harbor perspectives in biology · 2017Review
- Intermediate Filaments: Structure and Assembly.Cold Spring Harbor perspectives in biology · 2016Review
- Active multi-point microrheology of cytoskeletal networks.Beilstein journal of nanotechnology · 2016Article
- Mechanical Properties of Intermediate Filament Proteins.Methods in enzymology · 2016Article
- Mechanics of individual keratin bundles in living cells.Biophysical journal · 2014Article
- Attractive interactions among intermediate filaments determine network mechanics in vitro.PloS one · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanical properties of epithelial cells are modulated by structural changes in keratin intermediate filament networks. To investigate the relationship between network architecture and viscoelasticity, we assembled keratin filaments from recombinant keratin proteins 8 (K8) and 18 (K18) in the presence of divalent ions (Mg(2+)). We probed the viscoelastic modulus of the network by tracking the movement of microspheres embedded in the network during assembly, and studied the network architecture using scanning electron microscopy. Addition of Mg(2+) at physiological concentrations (<1 mM) resulted in networks whose structure was similar to that of keratin networks in epithelial cells. Moreover, the elastic moduli of networks assembled in vitro were found to be within the same magnitude as those measured in keratin networks of detergent-extracted epithelial cells. These findings suggest that Mg(2+)-induced filament cross-linking represents a valid model for studying the cytoskeletal mechanics of keratin networks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.