ArticleBMC medical genomics2012
The effects of MicroRNA transfections on global patterns of gene expression in ovarian cancer cells are functionally coordinated.
Article in BMC medical genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- miR-374a-5p regulates inflammatory genes and monocyte function in patients with inflammatory bowel disease.The Journal of experimental medicine · 2022Article
- Clinical significance of microRNA-125b and its contribution to ovarian carcinogenesis.Bioengineered · 2020Article
- Depletion of microRNA-451 in response to allergen exposure accentuates asthmatic inflammation by regulating Sirtuin2.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- High-throughput sequencing analysis revealed the regulation patterns of small RNAs on the development of A. comosus var. bracteatus leaves.Scientific reports · 2018Article
- MicroRNA-17 Suppresses TNF-α Signaling by Interfering with TRAF2 and cIAP2 Association in Rheumatoid Arthritis Synovial Fibroblasts.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Evidence for the role of microRNA 374b in acquired cisplatin resistance in pancreatic cancer cells.Cancer gene therapy · 2016Article
- Clinical Potential of microRNA-7 in Cancer.Journal of clinical medicine · 2015Review
- Key nodes of a microRNA network associated with the integrated mesenchymal subtype of high-grade serous ovarian cancer.Chinese journal of cancer · 2015Review
- Transcriptional override: a regulatory network model of indirect responses to modulations in microRNA expression.BMC systems biology · 2014Article
- MicroRNA expressions associated with progression of prostate cancer cells to antiandrogen therapy resistance.Molecular cancer · 2014Article
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- Epigenetic control of autophagy by microRNAs in ovarian cancer.BioMed research international · 2014Article
- Article
- Regulation of LH receptor mRNA binding protein by miR-122 in rat ovaries.Endocrinology · 2013Article
- MicroRNA-mediated regulation of target genes in several brain regions is correlated to both microRNA-targeting-specific promoter methylation and differential microRNA expression.BioData mining · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroRNAs (miRNAs) are a class of small RNAs that have been linked to a number of diseases including cancer. The potential application of miRNAs in the diagnostics and therapeutics of ovarian and other cancers is an area of intense interest. A current challenge is the inability to accurately predict the functional consequences of exogenous modulations in the levels of potentially therapeutic miRNAs.
methodsIn an initial effort to systematically address this issue, we conducted miRNA transfection experiments using two miRNAs (miR-7, miR-128). We monitored the consequent changes in global patterns of gene expression by microarray and quantitative (real-time) polymerase chain reaction. Network analysis of the expression data was used to predict the consequence of each transfection on cellular function and these predictions were experimentally tested.
resultsWhile ~20% of the changes in expression patterns of hundreds to thousands of genes could be attributed to direct miRNA-mRNA interactions, the majority of the changes are indirect, involving the downstream consequences of miRNA-mediated changes in regulatory gene expression. The changes in gene expression induced by individual miRNAs are functionally coordinated but distinct between the two miRNAs. MiR-7 transfection into ovarian cancer cells induces changes in cell adhesion and other developmental networks previously associated with epithelial-mesenchymal transitions (EMT) and other processes linked with metastasis. In contrast, miR-128 transfection induces changes in cell cycle control and other processes commonly linked with cellular replication.
conclusionsThe functionally coordinated patterns of gene expression displayed by different families of miRNAs have the potential to provide clinicians with a strategy to treat cancers from a systems rather than a single gene perspective.
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