ArticleMolecular endocrinology (Baltimore, Md.)2012
Stress levels of glucocorticoids inhibit LHβ-subunit gene expression in gonadotrope cells.
Article in Molecular endocrinology (Baltimore, Md.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 56 citations in OpenAlex.
- Injectable contraceptives differentially affect the hypothalamic-pituitary-gonadal axis and amenorrhea incidence†.Biology of reproduction · 2026Trial
- Omega-3 Fatty Acid Supplementation Lowers Serum FSH in Normal Weight But Not Obese Women.The Journal of clinical endocrinology and metabolism · 2016Trial
- Multiple Endocrine Disruption by the MET/ALK Inhibitor Crizotinib in Patients With Non-small Cell Lung Cancer.American journal of clinical oncology · 2015Trial
- HPA/HPG axes dysfunction: assessing the interrelations between diminished ovarian reserve and mental health.Journal of ovarian research · 2026Review
- Androgen and glucocorticoid crosstalk in granulosa cells: molecular insights into polyendocrine metabolic ovarian syndrome.Frontiers in endocrinology · 2026Review
- Stress and parental behaviors.Neuroscience research · 2025Review
- Predator scent stress reduces oxycodone self-administration and the nucleus accumbens dopamine response to oxycodone in female rats.Addiction neuroscience · 2025Article
- Impact of psychological stress on ovarian function: Insights, mechanisms and intervention strategies (Review).International journal of molecular medicine · 2025Review
- Longitudinal Evaluation of Reproductive Endocrine Function in Men With ACTH-Dependent Cushing Syndrome.The Journal of clinical endocrinology and metabolism · 2025Article
- Altered GnRH neuron and ovarian innervation characterize reproductive dysfunction linked to the Fragile X messenger ribonucleoprotein (Frontiers in endocrinology · 2023Article
- The Corticosterone-Glucocorticoid Receptor-AP1/CREB Axis Inhibits the Luteinizing Hormone Receptor Expression in Mouse Granulosa Cells.International journal of molecular sciences · 2022Article
- Review
- A Modified Ultra-Sensitive ELISA for Measurement of LH in Mice.Endocrinology · 2022Article
- Regulation of the gonadotropin-releasing hormone neuron during stress.Journal of neuroendocrinology · 2022Review
- Neuroendocrine interactions of the stress and reproductive axes.Frontiers in neuroendocrinology · 2021Review
- Unexpected Role of Physiological Estrogen in Acute Stress-Induced Memory Deficits.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Glucocorticoids affect male testicular steroidogenesis.Physiological research · 2020Review
- Estradiol Enables Chronic Corticosterone to Inhibit Pulsatile Luteinizing Hormone Secretion and Suppress Kiss1 Neuronal Activation in Female Mice.Neuroendocrinology · 2020Article
- Estrous Cycle Monitoring in Mice with Rapid Data Visualization and Analysis.Bio-protocol · 2019Article
- Emerging insights into hypothalamic-pituitary-gonadal axis regulation and interaction with stress signalling.Journal of neuroendocrinology · 2018Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Increased glucocorticoid secretion is a common response to stress and has been implicated as a mediator of reproductive suppression upon the pituitary gland. We utilized complementary in vitro and in vivo approaches in the mouse to investigate the role of glucocorticoids as a stress-induced intermediate capable of gonadotrope suppression. Repeated daily restraint stress lengthened the ovulatory cycle of female mice and acutely reduced GnRH-induced LH secretion and synthesis of LH β-subunit (LHβ) mRNA, coincident with increased circulating glucocorticoids. Administration of a stress level of glucocorticoid, in the absence of stress, blunted LH secretion in ovariectomized female mice, demonstrating direct impairment of reproductive function by glucocorticoids. Supporting a pituitary action, glucocorticoid receptor (GR) is expressed in mouse gonadotropes and treatment with glucocorticoids reduces GnRH-induced LHβ expression in immortalized mouse gonadotrope cells. Analyses revealed that glucocorticoid repression localizes to a region of the LHβ proximal promoter, which contains early growth response factor 1 (Egr1) and steroidogenic factor 1 sites critical for GnRH induction. GR is recruited to this promoter region in the presence of GnRH, but not by dexamethasone alone, confirming the necessity of the GnRH response for GR repression. In lieu of GnRH, Egr1 induction is sufficient for glucocorticoid repression of LHβ expression, which occurs via GR acting in a DNA- and dimerization-independent manner. Collectively, these results expose the gonadotrope as an important neuroendocrine site impaired during stress, by revealing a molecular mechanism involving Egr1 as a critical integrator of complex formation on the LHβ promoter during GnRH induction and GR repression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.