ArticleMolecular and cellular biology2012
Mediator promotes CENP-a incorporation at fission yeast centromeres.
Article in Molecular and cellular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Case Series of Nizon-Isidor Syndrome by Heterozygous Variants in MED12L With Further Evidence of Mitotic Instability in One Case With Diploid-Triploid Mosaicism.American journal of medical genetics. Part A · 2026Article
- Transcription-replication conflict resolution by nuclear RNA interference.Molecular cell · 2025Article
- The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis.Communications biology · 2022Article
- A role for MED14 and UVH6 in heterochromatin transcription upon destabilization of silencing.Life science alliance · 2018Article
- Centromere Biology: Transcription Goes on Stage.Molecular and cellular biology · 2018Review
- Generation of a Synthetic Human Chromosome with Two Centromeric Domains for Advanced Epigenetic Engineering Studies.ACS synthetic biology · 2018Article
- Mediator tail subunits can form amyloid-like aggregates in vivo and affect stress response in yeast.Nucleic acids research · 2015Article
- Epigenetic Regulation of Chromatin States in Schizosaccharomyces pombe.Cold Spring Harbor perspectives in biology · 2015Review
- RNA pol II transcript abundance controls condensin accumulation at mitotically up-regulated and heat-shock-inducible genes in fission yeast.Genes to cells : devoted to molecular & cellular mechanisms · 2015Article
- The Mediator complex: a central integrator of transcription.Nature reviews. Molecular cell biology · 2015Review
- Fluorescent probes for nucleic Acid visualization in fixed and live cells.Molecules (Basel, Switzerland) · 2013Review
- The head module of Mediator directs activation of preloaded RNAPII in vivo.Nucleic acids research · 2013Article
- The budding yeast point centromere associates with two Cse4 molecules during mitosis.Current biology : CB · 2013Article
- DNA topoisomerase III localizes to centromeres and affects centromeric CENP-A levels in fission yeast.PLoS genetics · 2013Article
- Mediator directs co-transcriptional heterochromatin assembly by RNA interference-dependent and -independent pathways.PLoS genetics · 2013Article
- Mediator regulates non-coding RNA transcription at fission yeast centromeres.Epigenetics & chromatin · 2012Article
- The Mediator complex and transcription regulation.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
At Schizosaccharomyces pombe centromeres, heterochromatin formation is required for de novo incorporation of the histone H3 variant CENP-A(Cnp1), which in turn directs kinetochore assembly and ultimately chromosome segregation during mitosis. Noncoding RNAs (ncRNAs) transcribed by RNA polymerase II (Pol II) directs heterochromatin formation through not only the RNA interference (RNAi) machinery but also RNAi-independent RNA processing factors. Control of centromeric ncRNA transcription is therefore a key factor for proper centromere function. We here demonstrate that Mediator directs ncRNA transcription and regulates centromeric heterochromatin formation in fission yeast. Mediator colocalizes with Pol II at centromeres, and loss of the Mediator subunit Med20 causes a dramatic increase in pericentromeric transcription and desilencing of the core centromere. As a consequence, heterochromatin formation is impaired via both the RNAi-dependent and -independent pathways, resulting in loss of CENP-A(Cnp1) from the core centromere, a defect in kinetochore function, and a severe chromosome segregation defect. Interestingly, the increased centromeric transcription observed in med20Δ cells appears to directly block CENP-A(Cnp1) incorporation since inhibition of Pol II transcription can suppress the observed phenotypes. Our data thus identify Mediator as a crucial regulator of ncRNA transcription at fission yeast centromeres and add another crucial layer of regulation to centromere function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.