Evidence map›Paper›PMID 22851529›Full record

ReviewAngewandte Chemie (International ed. in English)2012

Red fluorescent proteins: advanced imaging applications and future design.

Daria M Shcherbakova, Oksana M Subach, Vladislav V Verkhusha

Abstract readReview
In one paragraph

Review in Angewandte Chemie (International ed. in English), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.

0numbers the graph read from it
0cells of the map it votes in
69citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

69 citing papers in PubMed.

  1. Article
  2. Article
  3. A CandiChrome toolkit for multicolor labeling ofbioRxiv : the preprint server for biology · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. A fluorescent sex-sorting technique for insects with the demonstration inbioRxiv : the preprint server for biology · 2023
    Article
  13. Article
  14. An unusual disulfide-linked dimerization in the fluorescent protein rsCherryRev1.4.Acta crystallographica. Section F, Structural biology communications · 2023
    Article
  15. Review
  16. Article
  17. Choosing the Probe for Single-Molecule Fluorescence Microscopy.International journal of molecular sciences · 2022
    Review
  18. In Vivo Click Chemistry Enables Multiplexed Intravital Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022
    Article
  19. Cyanine Phototruncation Enables Spatiotemporal Cell Labeling.Journal of the American Chemical Society · 2022
    Article
  20. Review

9 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Daria M ShcherbakovaDepartment of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Oksana M Subach
Vladislav V Verkhusha

Funding

In Vivo Multiphoton Based Imaging of Complex Cancer Cell BehaviorR01CA164468 · NCI · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI VERKHUSHA, VLADISLAV · 2011 to 2015
$3.8M
Molecular Fluorescent ToolkitR01GM073913 · NIGMS · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI VERKHUSHA, VLADISLAV · 2006 to 2014
$2.4M
NCI NIH HHS CA164468NCI NIH HHS R01 CA164468NIGMS NIH HHS GM073913NIGMS NIH HHS R01 GM073913
6 · The paper itself

Abstract

In the past few years a large series of the advanced red-shifted fluorescent proteins (RFPs) has been developed. These enhanced RFPs provide new possibilities to study biological processes at the levels ranging from single molecules to whole organisms. Herein the relationship between the properties of the RFPs of different phenotypes and their applications to various imaging techniques are described. Existing and emerging imaging approaches are discussed for conventional RFPs, far-red FPs, RFPs with a large Stokes shift, fluorescent timers, irreversibly photoactivatable and reversibly photoswitchable RFPs. Advantages and limitations of specific RFPs for each technique are presented. Recent progress in understanding the chemical transformations of red chromophores allows the future RFP phenotypes and their respective novel imaging applications to be foreseen.

Indexed as

AnimalsBiosensing TechniquesFluorescence Resonance Energy TransferLuminescent ProteinsMicroscopyProtein EngineeringRed Fluorescent ProteinWhole Body ImagingLuminescent ProteinsRed Fluorescent Protein

Identifiers

PMID22851529
PMCPMC4433748

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.