ArticleThe Journal of biological chemistry2012
Nicotinic acid adenine dinucleotide phosphate (NAADP) degradation by alkaline phosphatase.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
- Tuning probe permeabilityChemical science · 2025Article
- Non-Target Metabolomics Reveals Changes in Metabolite Profiles in Distant Hybrid Incompatibility BetweenPlants (Basel, Switzerland) · 2025Article
- Enzymology of CaCells · 2023Review
- NAADP-Mediated CaHandbook of experimental pharmacology · 2023Article
- T cell CaFrontiers in immunology · 2023Review
- NAADP-Evoked CaHandbook of experimental pharmacology · 2023Article
- NAADP Signaling: New Kids on the Block.Cells · 2022Article
- Fluorogenic probes for imaging cellular phosphatase activity.Current opinion in chemical biology · 2020Review
- NAADP Receptors.Cold Spring Harbor perspectives in biology · 2019Review
- Integration of nicotinic acid adenine dinucleotide phosphate (NAADP)-dependent calcium signalling.The Journal of physiology · 2018Review
- 2'-Deoxyadenosine 5'-diphosphoribose is an endogenous TRPM2 superagonist.Nature chemical biology · 2017Article
- Integrated omics-analysis reveals Wnt-mediated NAD+ metabolic reprogramming in cancer stem-like cells.Oncotarget · 2016Article
- ADP-ribosyl cyclases regulate early development of the sea urchin.Messenger (Los Angeles, Calif. : Print) · 2016Article
- The enzymatic activities of CD38 enhance CLL growth and trafficking: implications for therapeutic targeting.Leukemia · 2015Article
- NAADP and the two-pore channel protein 1 participate in the acrosome reaction in mammalian spermatozoa.Molecular biology of the cell · 2014Article
- Nicotinic acid adenine dinucleotide phosphate (NAADP) is a second messenger in muscarinic receptor-induced contraction of guinea pig trachea.The Journal of biological chemistry · 2013Article
- Calcium pathway machinery at fertilization in echinoderms.Cell calcium · 2013Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a ubiquitous second messenger providing a Ca(2+) trigger in a wide range of cell types. However, its metabolism is not well understood. Here, we demonstrate the presence of endogenous NAADP in HeLa cells. CD38, a promiscuous enzyme described to be involved in NAADP metabolism, was not detectable in HeLa cells. In cell-free extracts of HeLa cells, NAADP was degraded to nicotinic acid adenine dinucleotide (NAAD). The enzyme was enriched in membranes (10,000 × g pellet) and displayed characteristics typical of alkaline phosphatase (AP), e.g. pH optimum at 8-9 and sensitivity to the inhibitors L-homoarginine and L-leucine. Importantly, NAADP at physiological concentrations (50-100 nM) was degraded to NAAD. Expression of AP isoenzymes was analyzed in HeLa cells. Based on the results together with inhibitor studies, the placental AP isoform emerged as the best candidate for NAADP degradation in HeLa cells. In contrast to HeLa cells, Jurkat T cells or HEK293 cells did not express any AP isoenzymes and did not display any NAADP 2'-phosphatase activity. Finally, the placental AP isoform was expressed heterologously in HEK293 cells, resulting in reconstitution of NAADP 2'-phosphatase activity in cell-free extracts. On the basis of the results, we provide evidence for AP as the metabolizing enzyme of NAADP in cells that do not express CD38.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.