ArticleJournal of industrial microbiology & biotechnology2012
Synthesis of methyl ketones by metabolically engineered Escherichia coli.
Article in Journal of industrial microbiology & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 50 citations in OpenAlex.
- Merging the computational design of chimeric type I polyketide synthases with enzymatic pathways for chemical biosynthesis.Nature communications · 2025Article
- Leveraging EngineeredACS synthetic biology · 2025Article
- Determinants of substrate specificity in a catalytically diverse family of acyl-ACP thioesterases from plants.BMC plant biology · 2023Article
- Synthetic metabolism forRSC chemical biology · 2022Article
- High-throughput colorimetric assays optimized for detection of ketones and aldehydes produced by microbial cell factories.Microbial biotechnology · 2022Article
- Metabolic engineering of β-oxidation to leverage thioesterases for production of 2-heptanone, 2-nonanone and 2-undecanone.Metabolic engineering · 2020Article
- Seasonal Effect on the Chemical Composition, Insecticidal Properties and Other Biological Activities ofFoods (Basel, Switzerland) · 2020Article
- Antioxidant and Lipoxygenase Inhibitory Activities of Essential Oils from Endemic Plants of Côte d'Ivoire:Molecules (Basel, Switzerland) · 2019Article
- Engineering E. coli for simultaneous glucose-xylose utilization during methyl ketone production.Microbial cell factories · 2018Article
- Engineering ofBiotechnology for biofuels · 2018Article
- Improving Escherichia coli membrane integrity and fatty acid production by expression tuning of FadL and OmpF.Microbial cell factories · 2017Article
- Biocide effects of volatile organic compounds produced by potential biocontrol rhizobacteria on Sclerotinia sclerotiorum.Frontiers in microbiology · 2015Article
- Heterologous expression of methylketone synthase1 and methylketone synthase2 leads to production of methylketones and myristic acid in transgenic plants.Plant physiology · 2014Article
- Fatty acid synthesis in Escherichia coli and its applications towards the production of fatty acid based biofuels.Biotechnology for biofuels · 2014Article
- Production of Fatty Acid-derived valuable chemicals in synthetic microbes.Frontiers in bioengineering and biotechnology · 2014Review
- Engineering of Ralstonia eutropha H16 for autotrophic and heterotrophic production of methyl ketones.Applied and environmental microbiology · 2013Article
- Next generation biofuel engineering in prokaryotes.Current opinion in chemical biology · 2013Review
- Polysaccharide hydrolysis with engineered Escherichia coli for the production of biocommodities.Journal of industrial microbiology & biotechnology · 2013Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methyl ketones are a group of highly reduced platform chemicals with widespread applications in the fragrance, flavor and pharmacological industries. Current methods for the industrial production of methyl ketones include oxidation of hydrocarbons, but recent advances in the characterization of methyl ketone synthases from wild tomato have sparked interest towards the development of microbial platforms for the industrial production of methyl ketones. A functional methyl ketone biosynthetic pathway was constructed in Escherichia coli by over-expressing two genes from Solanum habrochaites: shmks2, encoding a 3-ketoacyl-ACP thioesterase, and shmks1, encoding a beta-decarboxylase. These enzymes enabled methyl ketone synthesis from 3-ketoacyl-ACP, an intermediate in the fatty acid biosynthetic cycle. The production of 2-nonanone, 2-undecanone, and 2-tridecanone by MG1655 pTH-shmks2-shmks1 was initially detected by nuclear magnetic resonance and gas chromatography-mass spectrometry analyses at levels close to 6 mg/L. The deletion of major fermentative pathways leading to ethanol (adhE), lactate (ldhA), and acetate (pta, poxB) production allowed for the carbon flux to be redirected towards methyl ketone production, doubling total methyl ketone concentration. Variations in methyl ketone production observed under different working volumes in flask experiments led to a more detailed analysis of the effects of oxygen availability on methyl ketone concentration in order to determine optimal levels of oxygen. The methyl ketone concentration achieved with MG1655 ∆adhE ∆ldhA ∆poxB ∆pta pTrcHis2A-shmks2-shmks1, the best performer strain in this study, was approximately 500 mg/L, the highest reported for an engineered microorganism. Through the establishment of optimal operating conditions and by executing rational metabolic engineering strategies, we were able to increase methyl ketone concentrations by almost 75-fold from the initial confirmatory levels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.