ArticleThe EMBO journal2012
Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-EM.
Article in The EMBO journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Structure of human cytoplasmic Pol II complex explains global transcription repression by GDOWN1.Nature communications · 2026Article
- Structure of cytoplasmic RNA polymerase II.Nature communications · 2026Article
- Nuclear export restricts Gdown1 to a mitotic function.Nucleic acids research · 2022Article
- Structure of an inactive RNA polymerase II dimer.Nucleic acids research · 2021Article
- Regulation of hepatocyte cell cycle re-entry by RNA polymerase II-associated Gdown1.Cell cycle (Georgetown, Tex.) · 2020Review
- The plant Mediator complex and its role in jasmonate signaling.Journal of experimental botany · 2019Review
- Architecture of Pol II(G) and molecular mechanism of transcription regulation by Gdown1.Nature structural & molecular biology · 2018Article
- Born to run: control of transcription elongation by RNA polymerase II.Nature reviews. Molecular cell biology · 2018Review
- Approaches to altering particle distributions in cryo-electron microscopy sample preparation.Acta crystallographica. Section D, Structural biology · 2018Review
- Super elongation complex contains a TFIIF-related subcomplex.Transcription · 2016Article
- Article
- The young person's guide to the PDB.Postepy biochemii · 2016Review
- Gdown1 Associates Efficiently with RNA Polymerase II after Promoter Clearance and Displaces TFIIF during Transcript Elongation.PloS one · 2016Article
- The Mediator complex: a central integrator of transcription.Nature reviews. Molecular cell biology · 2015Review
- Regulation of RNA polymerase II termination by phosphorylation of Gdown1.The Journal of biological chemistry · 2014Article
- Functional interactions of the RNA polymerase II-interacting proteins Gdown1 and TFIIF.The Journal of biological chemistry · 2014Article
- Structural interactions between inhibitor and substrate docking sites give insight into mechanisms of human PS1 complexes.Structure (London, England : 1993) · 2014Article
- The RNA polymerase II preinitiation complex. Through what pathway is the complex assembled?Transcription · 2014Review
- RNA polymerase II transcription elongation control.Chemical reviews · 2013Review
- The Spt4-Spt5 complex: a multi-faceted regulator of transcription elongation.Biochimica et biophysica acta · 2013Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
In mammals, a distinct RNA polymerase II form, RNAPII(G) contains a novel subunit Gdown1 (encoded by POLR2M), which represses gene activation, only to be reversed by the multisubunit Mediator co-activator. Here, we employed single-particle cryo-electron microscopy (cryo-EM) to disclose the architectures of RNAPII(G), RNAPII and RNAPII in complex with the transcription initiation factor TFIIF, all to ~19 Å. Difference analysis mapped Gdown1 mostly to the RNAPII Rpb5 shelf-Rpb1 jaw, supported by antibody labelling experiments. These structural features correlate with the moderate increase in the efficiency of RNA chain elongation by RNAP II(G). In addition, our updated RNAPII-TFIIF map showed that TFIIF tethers multiple regions surrounding the DNA-binding cleft, in agreement with cross-linking and biochemical mapping. Gdown1's binding sites overlap extensively with those of TFIIF, with Gdown1 sterically excluding TFIIF from RNAPII, herein demonstrated by competition assays using size exclusion chromatography. In summary, our work establishes a structural basis for Gdown1 impeding initiation at promoters, by obstruction of TFIIF, accounting for an additional dependent role of Mediator in activated transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.