ArticleThe EMBO journal2012
Arabidopsis Argonaute MID domains use their nucleotide specificity loop to sort small RNAs.
Article in The EMBO journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 103 citations in OpenAlex.
- Decoding Argonaute specificity: insights fromRNA (New York, N.Y.) · 2026Review
- Plant microRNA maturation and function.Nature reviews. Molecular cell biology · 2026Review
- Arabidopsis AGO1 N-terminal extension acts as an essential hub for PRMT5 interaction and post-translational modifications.Nucleic acids research · 2024Article
- Challenges and Opportunities Arising from Host-International journal of molecular sciences · 2024Review
- The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein.Nucleic acids research · 2024Article
- Barley AGO4 proteins show overlapping functionality with distinct small RNA-binding properties in heterologous complementation.Plant cell reports · 2024Article
- Small RNAs >26 nt in length associate with AGO1 and are upregulated by nutrient deprivation in the alga Chlamydomonas.The Plant cell · 2023Article
- Structural basis for sequence-specific recognition of guide and target strands by the Archaeoglobus fulgidus Argonaute protein.Scientific reports · 2023Article
- The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities.PLoS genetics · 2023Article
- Enzymatic reactions of AGO4 in RNA-directed DNA methylation: siRNA duplex loading, passenger strand elimination, target RNA slicing, and sliced target retention.Genes & development · 2023Article
- The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation.Nucleic acids research · 2022Article
- The molecular mechanism of microRNA duplex selectivity of Arabidopsis ARGONAUTE10.Nucleic acids research · 2022Article
- Origin, evolution and diversification of plant ARGONAUTE proteins.The Plant journal : for cell and molecular biology · 2022Article
- Argonaute proteins: structures and their endonuclease activity.Molecular biology reports · 2021Review
- Prokaryotic Argonaute from Archaeoglobus fulgidus interacts with DNA as a homodimer.Scientific reports · 2021Article
- Beyond identity: Understanding the contribution of the 5' nucleotide of the antisense strand to RNAi activity.PloS one · 2021Article
- A Hyperthermophilic Argonaute FromFrontiers in microbiology · 2021Article
- Genome-wide identification of Argonautes in Solanaceae with emphasis on potato.Scientific reports · 2020Article
- Abundant expression of maternal siRNAs is a conserved feature of seed development.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Genome-Wide Identification of RNA Silencing-Related Genes and Their Expressional Analysis in Response to Heat Stress in Barley (Biomolecules · 2020Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The 5'-nucleotide of small RNAs associates directly with the MID domain of Argonaute (AGO) proteins. In humans, the identity of the 5'-base is sensed by the MID domain nucleotide specificity loop and regulates the integrity of miRNAs. In Arabidopsis thaliana, the 5'-nucleotide also controls sorting of small RNAs into the appropriate member of the AGO family; however, the structural basis for this mechanism is unknown. Here, we present crystal structures of the MID domain from three Arabidopsis AGOs, AtAGO1, AtAGO2 and AtAGO5, and characterize their interactions with nucleoside monophosphates (NMPs). In AtAGOs, the nucleotide specificity loop also senses the identity of the 5'-nucleotide but uses more diverse modes of recognition owing to the greater complexity of small RNAs found in plants. Binding analyses of these interactions reveal a strong correlation between their affinities and evolutionary conservation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.