ArticlePloS one2012
454 Transcriptome sequencing suggests a role for two-component signalling in cellularization and differentiation of barley endosperm transfer cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Genome-wide characterization of two-component system elements in barley enables the identification of grain-specific phosphorelay genes.BMC plant biology · 2025Article
- Dissection of Developmental Programs and Regulatory Modules Directing Endosperm Transfer Cell and Aleurone Identity in the Syncytial Endosperm of Barley.Plants (Basel, Switzerland) · 2023Article
- Distinct Hormone Signalling-Modulation Activities Characterize Two Maize Endosperm-Specific Type-A Response Regulators.Plants (Basel, Switzerland) · 2022Article
- Molecular mechanisms of maize endosperm transfer cell development.Plant cell reports · 2022Review
- Maize unstable factor for orange1 is essential for endosperm development and carbohydrate accumulation.Plant physiology · 2021Article
- The Dynamics of Transcript Abundance during Cellularization of Developing Barley Endosperm.Plant physiology · 2016Article
- Identification of Candidate Transcriptional Regulators of Epidermal Transfer Cell Development in Vicia faba Cotyledons.Frontiers in plant science · 2016Article
- Differential transcriptional networks associated with key phases of ingrowth wall construction in trans-differentiating epidermal transfer cells of Vicia faba cotyledons.BMC plant biology · 2015Article
- Metabolic and transcriptional transitions in barley glumes reveal a role as transitory resource buffers during endosperm filling.Journal of experimental botany · 2015Article
- Expression patterns and protein structure of a lipid transfer protein END1 from Arabidopsis.Planta · 2014Article
- Transcriptome sequencing of two wild barley (Hordeum spontaneum L.) ecotypes differentially adapted to drought stress reveals ecotype-specific transcripts.BMC genomics · 2014Article
- Phenotypic and Transcriptional Analysis of Divergently Selected Maize Populations Reveals the Role of Developmental Timing in Seed Size Determination.Plant physiology · 2014Article
- Evolution and development of cell walls in cereal grains.Frontiers in plant science · 2014Review
- Caspase-like activities accompany programmed cell death events in developing barley grains.PloS one · 2014Article
- WHIRLY1 is a major organizer of chloroplast nucleoids.Frontiers in plant science · 2014Article
- Development of endosperm transfer cells in barley.Frontiers in plant science · 2014Review
- Transcriptomic signatures of transfer cells in early developing nematode feeding cells of Arabidopsis focused on auxin and ethylene signaling.Frontiers in plant science · 2014Review
- Endosperm transfer cell-specific genes and proteins: structure, function and applications in biotechnology.Frontiers in plant science · 2014Review
- Intersection of transfer cells with phloem biology-broad evolutionary trends, function, and induction.Frontiers in plant science · 2013Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCell specification and differentiation in the endosperm of cereals starts at the maternal-filial boundary and generates the endosperm transfer cells (ETCs). Besides the importance in assimilate transfer, ETCs are proposed to play an essential role in the regulation of endosperm differentiation by affecting development of proximate endosperm tissues. We attempted to identify signalling elements involved in early endosperm differentiation by using a combination of laser-assisted microdissection and 454 transcriptome sequencing. PRINCIPAL
findings454 sequencing of the differentiating ETC region from the syncytial state until functionality in transfer processes captured a high proportion of novel transcripts which are not available in existing barley EST databases. Intriguingly, the ETC-transcriptome showed a high abundance of elements of the two-component signalling (TCS) system suggesting an outstanding role in ETC differentiation. All components and subfamilies of the TCS, including distinct kinds of membrane-bound receptors, have been identified to be expressed in ETCs. The TCS system represents an ancient signal transduction system firstly discovered in bacteria and has previously been shown to be co-opted by eukaryotes, like fungi and plants, whereas in animals and humans this signalling route does not exist. Transcript profiling of TCS elements by qRT-PCR suggested pivotal roles for specific phosphorelays activated in a coordinated time flow during ETC cellularization and differentiation. ETC-specificity of transcriptionally activated TCS phosphorelays was assessed for early differentiation and cellularization contrasting to an extension of expression to other grain tissues at the beginning of ETC maturation. Features of candidate genes of distinct phosphorelays and transcriptional activation of genes putatively implicated in hormone signalling pathways hint at a crosstalk of hormonal influences, putatively ABA and ethylene, and TCS signalling. SIGNIFICANCE: Our findings suggest an integral function for the TCS in ETC differentiation possibly coupled to sequent hormonal regulation by ABA and ethylene.
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