ArticlePloS one2012
Two-component systems are involved in the regulation of botulinum neurotoxin synthesis in Clostridium botulinum type A strain Hall.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Elucidating the activation mechanism of botulinum neurotoxin a: role of α-clostripain and NTNH.Scientific reports · 2025Article
- Review
- Time-course transcriptomics reveals that amino acids catabolism plays a key role in toxinogenesis and morphology in Clostridium tetani.Journal of industrial microbiology & biotechnology · 2020Article
- Tetanus Toxin Synthesis is Under the Control of A Complex Network of Regulatory Genes inToxins · 2020Article
- Regulation of Botulinum Neurotoxin Synthesis and Toxin Complex Formation by Arginine and Glucose in Clostridium botulinum ATCC 3502.Applied and environmental microbiology · 2017Article
- Article
- An Integrative Approach to Computational Modelling of the Gene Regulatory Network Controlling Clostridium botulinum Type A1 Toxin Production.PLoS computational biology · 2016Article
- New Elements To Consider When Modeling the Hazards Associated with Botulinum Neurotoxin in Food.Journal of bacteriology · 2016Review
- The pattern of growth observed for Clostridium botulinum type A1 strain ATCC 19397 is influenced by nutritional status and quorum sensing: a modelling perspective.Pathogens and disease · 2015Article
- Virulence Plasmids of Spore-Forming Bacteria.Microbiology spectrum · 2014Review
- Positive regulation of botulinum neurotoxin gene expression by CodY in Clostridium botulinum ATCC 3502.Applied and environmental microbiology · 2014Article
- The cold-induced two-component system CBO0366/CBO0365 regulates metabolic pathways with novel roles in group I Clostridium botulinum ATCC 3502 cold tolerance.Applied and environmental microbiology · 2014Article
- Comparison of assembled Clostridium botulinum A1 genomes revealed their evolutionary relationship.Genomics · 2014Article
- Two-component signal transduction system CBO0787/CBO0786 represses transcription from botulinum neurotoxin promoters in Clostridium botulinum ATCC 3502.PLoS pathogens · 2013Article
- Molecular composition and extinction coefficient of native botulinum neurotoxin complex produced by Clostridium botulinum hall A strain.The protein journal · 2013Article
- Structure and genetic content of the megaplasmids of neurotoxigenic clostridium butyricum type E strains from Italy.PloS one · 2013Article
- Antisense RNA regulation and application in the development of novel antibiotics to combat multidrug resistant bacteria.Science progress · 2013Review
Corrections and comments
- Erratum issuedBrueggemann, Holger [corrected to Brüggemann, Holger]
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium botulinum synthesizes a potent neurotoxin (BoNT) which associates with non-toxic proteins (ANTPs) to form complexes of various sizes. The bont and antp genes are clustered in two operons. In C. botulinum type A, bont/A and antp genes are expressed during the end of the exponential growth phase and the beginning of the stationary phase under the control of an alternative sigma factor encoded by botR/A, which is located between the two operons. In the genome of C. botulinum type A strain Hall, 30 gene pairs predicted to encode two-component systems (TCSs) and 9 orphan regulatory genes have been identified. Therefore, 34 Hall isogenic antisense strains on predicted regulatory genes (29 TCSs and 5 orphan regulatory genes) have been obtained by a mRNA antisense procedure. Two TCS isogenic antisense strains showed more rapid growth kinetics and reduced BoNT/A production than the control strain, as well as increased bacterial lysis and impairment of the bacterial cell wall structure. Three other TCS isogenic antisense strains induced a low level of BoNT/A and ANTP production. Interestingly, reduced expression of bont/A and antp genes was shown to be independent of botR/A. These results indicate that BoNT/A synthesis is under the control of a complex network of regulation including directly at least three TCSs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.