ArticlePloS one2012
A BCAM0223 mutant of Burkholderia cenocepacia is deficient in hemagglutination, serum resistance, adhesion to epithelial cells and virulence.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Quantitative Imaging of the Action of vCPP2319, an Antimicrobial Peptide from a Viral Scaffold, againstACS infectious diseases · 2023Article
- Antibacterial Activity of ZnO Nanoparticles in aJournal of functional biomaterials · 2023Article
- In-Silico Analysis Highlights the Existence in Members ofMicroorganisms · 2023Article
- A designed cyclic analogue of gomesin has potent activity against Staphylococcus aureus biofilms.The Journal of antimicrobial chemotherapy · 2022Article
- Burkholderia cenocepacia transcriptome during the early contacts with giant plasma membrane vesicles derived from live bronchial epithelial cells.Scientific reports · 2021Article
- Role of CgTpo4 in Polyamine and Antimicrobial Peptide Resistance: Determining Virulence inInternational journal of molecular sciences · 2021Article
- Coaggregation properties of trimeric autotransporter adhesins.MicrobiologyOpen · 2020Article
- Burkholderia cenocepacia-host cell contact controls the transcription activity of the trimeric autotransporter adhesin BCAM2418 gene.MicrobiologyOpen · 2020Article
- The Mla Pathway Plays an Essential Role in the Intrinsic Resistance of Burkholderia cepacia Complex Species to Antimicrobials and Host Innate Components.Journal of bacteriology · 2018Article
- The involvement of the low-oxygen-activated locus of Burkholderia cenocepacia in adaptation during cystic fibrosis infection.Scientific reports · 2018Article
- Repair of Iron Centers RIC protein contributes to the virulence of Staphylococcus aureus.Virulence · 2018Article
- A New Determinant ofFrontiers in cellular and infection microbiology · 2017Article
- Prospects for subunit vaccines: Technology advances resulting in efficacious antigens requires matching advances in early clinical trial investment.Human vaccines & immunotherapeutics · 2016Article
- Linocin and OmpW Are Involved in Attachment of the Cystic Fibrosis-Associated Pathogen Burkholderia cepacia Complex to Lung Epithelial Cells and Protect Mice against Infection.Infection and immunity · 2016Article
- Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity.Frontiers in microbiology · 2016Article
- The tyrosine kinase BceF and the phosphotyrosine phosphatase BceD of Burkholderia contaminans are required for efficient invasion and epithelial disruption of a cystic fibrosis lung epithelial cell line.Infection and immunity · 2015Article
- Mechanisms of Disease: Host-Pathogen Interactions between Burkholderia Species and Lung Epithelial Cells.Frontiers in cellular and infection microbiology · 2015Review
- Article
- Characterization of BCAM0224, a multifunctional trimeric autotransporter from the human pathogen Burkholderia cenocepacia.Journal of bacteriology · 2014Article
- Functional characterization of Burkholderia pseudomallei trimeric autotransporters.Infection and immunity · 2013Article
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2 authors.
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Abstract
Burkholderia cepacia complex (Bcc) bacteria are a problematic group of microorganisms causing severe infections in patients with Cystic Fibrosis. In early stages of infection, Bcc bacteria must be able to adhere to and colonize the respiratory epithelium. Although this is not fully understood, this primary stage of infection is believed to be in part mediated by a specific type of adhesins, named trimeric autotransporter adhesins (TAAs). These homotrimeric proteins exist on the surface of many gram negative pathogens and often mediate a number of critical functions, including biofilm formation, serum resistance and adherence to an invasion of host cells. We have previously identified in the genome of the epidemic clinical isolate B. cenocepacia J2315, a novel cluster of genes putatively encoding three TAAs (BCAM0219, BCAM0223 and BCAM0224). In this study, the genomic organization of the TAA cluster has been determined. To further address the direct role of the putative TAA BCAM0223 in B. cenocepacia pathogenicity, an isogenic mutant was constructed via insertional inactivation. The BCAM0223::Tp mutant is deficient in hemagglutination, affected in adherence to vitronectin and in biofilm formation and showed attenuated virulence in the Galleria mellonella model of infection. Moreover, the BCAM0223::Tp mutant also showed a significant reduction in its resistance to human serum as well as in adherence, but not in invasion of, cultured human bronchial epithelial cells. Altogether these results demonstrate that the BCAM0223 protein is a multifunctional virulence factor that may contribute to the pathogenicity of B. cenocepacia.
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