ArticlePloS one2012
Investigating the production of foreign membrane proteins in tobacco chloroplasts: expression of an algal plastid terminal oxidase.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Salt-Induced Modulation of Ion Transport and PSII Photoprotection Determine the Salinity Tolerance of Amphidiploid Brassicas.Plants (Basel, Switzerland) · 2023Article
- Photosynthetic machinery under salinity stress: Trepidations and adaptive mechanisms.Photosynthetica · 2023Review
- Plastid Transformation: New Challenges in the Circular Economy Era.International journal of molecular sciences · 2022Review
- PTOX-dependent safety valve does not oxidize P700 during photosynthetic induction in the Arabidopsis pgr5 mutant.Plant physiology · 2022Article
- Here comes the sun: How optimization of photosynthetic light reactions can boost crop yields.Journal of integrative plant biology · 2022Review
- Review
- The evolutionary conserved iron-sulfur protein TCR controls P700 oxidation in photosystem I.iScience · 2021Article
- Contrasting Responses of Plastid Terminal Oxidase Activity Under Salt Stress in Two CFrontiers in plant science · 2020Article
- Contrasting Responses to Stress Displayed by Tobacco Overexpressing an Algal Plastid Terminal Oxidase in the Chloroplast.Frontiers in plant science · 2020Article
- Article
- Overexpression of plastid terminal oxidase inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Article
- Impaired Mitochondrial Transcription Termination Disrupts the Stromal Redox Poise inPlant physiology · 2017Article
- Recent achievements obtained by chloroplast transformation.Plant methods · 2017Review
- Phaseolin expression in tobacco chloroplast reveals an autoregulatory mechanism in heterologous protein translation.Plant biotechnology journal · 2016Article
- Testing the Role of the N-Terminal Tail of D1 in the Maintenance of Photosystem II in Tobacco Chloroplasts.Frontiers in plant science · 2016Article
- The Dual Role of the Plastid Terminal Oxidase PTOX: Between a Protective and a Pro-oxidant Function.Frontiers in plant science · 2015Article
- Transplastomic integration of a cyanobacterial bicarbonate transporter into tobacco chloroplasts.Journal of experimental botany · 2014Article
- Regulation of photosynthetic electron transport and photoinhibition.Current protein & peptide science · 2014Review
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplast transformation provides an inexpensive, easily scalable production platform for expression of recombinant proteins in plants. However, this technology has been largely limited to the production of soluble proteins. Here we have tested the ability of tobacco chloroplasts to express a membrane protein, namely plastid terminal oxidase 1 from the green alga Chlamydomonas reinhardtii (Cr-PTOX1), which is predicted to function as a plastoquinol oxidase. A homoplastomic plant containing a codon-optimised version of the nuclear gene encoding PTOX1, driven by the 16S rRNA promoter and 5'UTR of gene 10 from phage T7, was generated using a particle delivery system. Accumulation of Cr-PTOX1 was shown by immunoblotting and expression in an enzymatically active form was confirmed by using chlorophyll fluorescence to measure changes in the redox state of the plastoquinone pool in leaves. Growth of Cr-PTOX1 expressing plants was, however, more sensitive to high light than WT. Overall our results confirm the feasibility of using plastid transformation as a means of expressing foreign membrane proteins in the chloroplast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.