ArticlePloS one2012
Expression and stability of foreign epitopes introduced into 3A nonstructural protein of foot-and-mouth disease virus.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- The La Region of Foot-and-Mouth Disease Virus: Essential for L Protein Cellular Distribution but Not Functional Activity.International journal of molecular sciences · 2026Article
- Development of solid phase competitive enzyme-linked immunosorbent assay to detect foot-and-mouth disease virus A serotype specific antibodies based on immunoglobulin Y antibody.Veterinary research forum : an international quarterly journal · 2025Article
- Modification of the second translation initiation site restricts the replication of foot-and-mouth disease virus in PK-15 cells.Applied microbiology and biotechnology · 2020Article
- Cellular Vimentin Interacts with Foot-and-Mouth Disease Virus Nonstructural Protein 3A and Negatively Modulates Viral Replication.Journal of virology · 2020Article
- Engineering viable foot-and-mouth disease viruses with increased acid stability facilitate the development of improved vaccines.Applied microbiology and biotechnology · 2020Article
- Article
- Article
- Antigenic properties and virulence of foot-and-mouth disease virus rescued from full-length cDNA clone of serotype O, typical vaccine strain.Clinical and experimental vaccine research · 2015Article
- Evaluation of a 3A-truncated foot-and-mouth disease virus in pigs for its potential as a marker vaccine.Veterinary research · 2014Article
- Requirements for improved vaccines against foot-and-mouth disease epidemics.Clinical and experimental vaccine research · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Foot-and-mouth disease virus (FMDV) is an aphthovirus that belongs to the Picornaviridae family and causes one of the most important animal diseases worldwide. The capacity of other picornaviruses to express foreign antigens has been extensively reported, however, little is known about FMDV. To explore the potential of FMDV as a viral vector, an 11-amino-acid (aa) HSV epitope and an 8 aa FLAG epitope were introduced into the C-terminal different regions of 3A protein of FMDV full-length infectious cDNA clone. Recombinant viruses expressing the HSV or FLAG epitope were successfully rescued after transfection of both modified constructs. Immunofluorescence assay, Western blot and sequence analysis showed that the recombinant viruses stably maintained the foreign epitopes even after 11 serial passages in BHK-21 cells. The 3A-tagged viruses shared similar plaque phenotypes and replication kinetics to those of the parental virus. In addition, mice experimentally infected with the epitope-tagged viruses could induce tag-specific antibodies. Our results demonstrate that FMDV can be used effectively as a viral vector for the delivery of foreign tags.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.