ArticlePloS one2012
Automated screening of microtubule growth dynamics identifies MARK2 as a regulator of leading edge microtubules downstream of Rac1 in migrating cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- MARK2 regulates Golgi apparatus reorientation by phosphorylation of CAMSAP2 in directional cell migratio.eLife · 2025Article
- Role of MARK2 in the nervous system and cancer.Cancer gene therapy · 2024Review
- MACC1-induced migration in tumors: Current state and perspective.Frontiers in oncology · 2023Review
- MARK2 and MARK4 Regulate Sertoli Cell BTB Dynamics Through Microtubule and Actin Cytoskeletons.Endocrinology · 2022Article
- MARK2 regulates directed cell migration through modulation of myosin II contractility and focal adhesion organization.Current biology : CB · 2022Article
- Microtubules Regulate Localization and Availability of Insulin Granules in Pancreatic Beta Cells.Biophysical journal · 2020Article
- MARK2/Par1b kinase present at centrosomes and retraction fibres corrects spindle off-centring induced by actin disassembly.Open biology · 2019Article
- Spindle rotation in human cells is reliant on a MARK2-mediated equatorial spindle-centering mechanism.The Journal of cell biology · 2018Article
- Neuronal Growth Cone Size-Dependent and -Independent Parameters of Microtubule Polymerization.Frontiers in cellular neuroscience · 2018Article
- PAR proteins regulate maintenance-phase myosin dynamics duringMolecular biology of the cell · 2017Article
- Fidgetin regulates cultured astrocyte migration by severing tyrosinated microtubules at the leading edge.Molecular biology of the cell · 2017Article
- The spectraplakin Short stop is an essential microtubule regulator involved in epithelial closure inJournal of cell science · 2017Article
- Extracting microtubule networks from superresolution single-molecule localization microscopy data.Molecular biology of the cell · 2017Article
- MARK2 Rescues Nogo-66-Induced Inhibition of Neurite Outgrowth via Regulating Microtubule-Associated Proteins in Neurons In Vitro.Neurochemical research · 2016Article
- Regulation of human genome expression and RNA splicing by human papillomavirus 16 E2 protein.Virology · 2014Article
- Using plusTipTracker software to measure microtubule dynamics in Xenopus laevis growth cones.Journal of visualized experiments : JoVE · 2014Article
- Communication, the centrosome and the immunological synapse.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Rac1 and Aurora A regulate MCAK to polarize microtubule growth in migrating endothelial cells.The Journal of cell biology · 2014Article
- Cep192 controls the balance of centrosome and non-centrosomal microtubules during interphase.PloS one · 2014Article
- Hedgehog signaling controls T cell killing at the immunological synapse.Science (New York, N.Y.) · 2013Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
Polarized microtubule (MT) growth in the leading edge is critical to directed cell migration, and is mediated by Rac1 GTPase. To find downstream targets of Rac1 that affect MT assembly dynamics, we performed an RNAi screen of 23 MT binding and regulatory factors and identified RNAi treatments that suppressed changes in MT dynamics induced by constitutively activated Rac1. By analyzing fluorescent EB3 dynamics with automated tracking, we found that RNAi treatments targeting p150(glued), APC2, spastin, EB1, Op18, or MARK2 blocked Rac1-mediated MT growth in lamellipodia. MARK2 was the only protein whose RNAi targeting additionally suppressed Rac1 effects on MT orientation in lamellipodia, and thus became the focus of further study. We show that GFP-MARK2 rescued effects of MARK2 depletion on MT growth lifetime and orientation, and GFP-MARK2 localized in lamellipodia in a Rac1-activity-dependent manner. In a wound-edge motility assay, MARK2-depleted cells failed to polarize their centrosomes or exhibit oriented MT growth in the leading edge, and displayed defects in directional cell migration. Thus, automated image analysis of MT assembly dynamics identified MARK2 as a target regulated downstream of Rac1 that promotes oriented MT growth in the leading edge to mediate directed cell migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.