Trial reportPloS one2012
Trypanosoma cruzi IV causing outbreaks of acute Chagas disease and infections by different haplotypes in the Western Brazilian Amazonia.
Trial report in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 91 citations in OpenAlex.
- Orally transmitted Chagas disease outbreaks in Latin America: a comprehensive systematic review and public health implications.Frontiers in public health · 2026Pooled it
- Article
- Trypanosoma cruzi DTU parasite diversity and clinical outcomes in mesoregions of the Northeast Brazilian State of Pernambuco.PLoS neglected tropical diseases · 2026Article
- Overview of Chagas disease in Amazonas: an integrated approach.Revista da Sociedade Brasileira de Medicina Tropical · 2026Review
- Genetic diversity of Trypanosoma cruzi in individuals with chronic Chagas disease in the Northern Minas Gerais and Vale do Jequitinhonha regions, Minas Gerais, Brazil.PLoS neglected tropical diseases · 2025Article
- Trypanosoma cruzi: A new system for primary culture and isolation of the parasite.Parasitology research · 2025Article
- Chronic Chagas Disease in the Brazilian Amazon: Serological Survey, Clinical Follow-Up, and Associated Risk Factors.The American journal of tropical medicine and hygiene · 2025Article
- Acute Chagas disease in Amazonia, western Pará: perspectives from medical assistance to genetic elucidation.Memorias do Instituto Oswaldo Cruz · 2025Observational
- Intranasal Trans-Sialidase Vaccine Mitigates Acute and Chronic Pathology in a Preclinical Oral Chagas Disease Model.Vaccines · 2024Article
- Exploring Trypanosoma cruzi transmission dynamics in an acute Chagas disease outbreak using next-generation sequencing.Parasites & vectors · 2024Article
- Comparative analysis of metacyclogenesis and infection curves in different discrete typing units of Trypanosoma cruzi.Parasitology research · 2024Article
- New insights intoFrontiers in immunology · 2024Review
- Fifteen Years after the Definition ofLife (Basel, Switzerland) · 2023Review
- Detection of Trypanosoma cruzi DTUs TcI and TcIV in two outbreaks of orally-transmitted Chagas disease in the Northern region of Brazil.Revista do Instituto de Medicina Tropical de Sao Paulo · 2023Article
- Chronic Chagas Cardiomyopathy in the Brazilian Amazon region: clinical characteristics and regional distinctiveness.Frontiers in public health · 2023Review
- Article
- Molecular characterization ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Triatoma williami in intradomiciliary environments of urban areas in Mato Grosso State, Brazil: domiciliation process of a wild species?Infectious diseases of poverty · 2022Article
- Maxicircle architecture and evolutionary insights into Trypanosoma cruzi complex.PLoS neglected tropical diseases · 2021Article
- The Selvester QRS score as an estimative of myocardial injury in acute chagasic patients from the Brazilian Amazon.BMC infectious diseases · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChagas disease is an emergent tropical disease in the Brazilian Amazon Region, with an increasing number of cases in recent decades. In this region, the sylvatic cycle of Trypanosoma cruzi transmission, which constitutes a reservoir of parasites that might be associated with specific molecular, epidemiological and clinical traits, has been little explored. The objective of this work is to genetically characterize stocks of T. cruzi from human cases, triatomines and reservoir mammals in the State of Amazonas, in the Western Brazilian Amazon. METHODOLOGY/PRINCIPAL
findingsWe analyzed 96 T. cruzi samples from four municipalities in distant locations of the State of Amazonas. Molecular characterization of isolated parasites from cultures in LIT medium or directly from vectors or whole human blood was performed by PCR of the non-transcribed spacer of the mini-exon and of the 24 S alfa ribosomal RNA gene, RFLP and sequencing of the mitochondrial cytochrome c oxidase subunit II (COII) gene, and by sequencing of the glucose-phosphate isomerase gene. The T. cruzi parasites from two outbreaks of acute disease were all typed as TcIV. One of the outbreaks was triggered by several haplotypes of the same DTU. TcIV also occurred in isolated cases and in Rhodnius robustus. Incongruence between mitochondrial and nuclear phylogenies is likely to be indicative of historical genetic exchange events resulting in mitochondrial introgression between TcIII and TcIV DTUs from Western Brazilian Amazon. TcI predominated among triatomines and was the unique DTU infecting marsupials. CONCLUSION/SIGNIFICANCE: DTU TcIV, rarely associated with human Chagas disease in other areas of the Amazon basin, is the major strain responsible for the human infections in the Western Brazilian Amazon, occurring in outbreaks as single or mixed infections by different haplotypes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.