ArticlePloS one2012
Detecting the immune system response of a 500 year-old Inca mummy.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Sequential trypsin and ProAlanase digestions unearth immunological protein biomarkers shrouded by skeletal collagen.iScience · 2024Article
- Paleoproteomics.Chemical reviews · 2022Review
- Ancient protein analysis in archaeology.Science advances · 2021Review
- Palaeoproteomic identification of breast milk protein residues from the archaeological skeletal remains of a neonatal dog.Scientific reports · 2019Article
- Proteomic profiling of archaeological human bone.Royal Society open science · 2017Article
- Resurrecting the Dead (Molecules).Computational and structural biotechnology journal · 2017Review
- Paleoproteomics of the Dental Pulp: The plague paradigm.PloS one · 2017Article
- Preservation of the metaproteome: variability of protein preservation in ancient dental calculus.Science and technology of archaeological research · 2017Article
- Identification of proteins from 4200-year-old skin and muscle tissue biopsies from ancient Egyptian mummies of the first intermediate period shows evidence of acute inflammation and severe immune response.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2016Article
- Natural mummification of the human gut preserves bacteriophage DNA.FEMS microbiology letters · 2016Article
- Ancient human microbiomes.Journal of human evolution · 2015Article
- A new era in palaeomicrobiology: prospects for ancient dental calculus as a long-term record of the human oral microbiome.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Invasive versus Non Invasive Methods Applied to Mummy Research: Will This Controversy Ever Be Solved?BioMed research international · 2015Review
- Circulating Mycobacterium bovis peptides and host response proteins as biomarkers for unambiguous detection of subclinical infection.Journal of clinical microbiology · 2014Article
- Archaeological, radiological, and biological evidence offer insight into Inca child sacrifice.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- First insights into the metagenome of Egyptian mummies using next-generation sequencing.Journal of applied genetics · 2013Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Disease detection in historical samples currently relies on DNA extraction and amplification, or immunoassays. These techniques only establish pathogen presence rather than active disease. We report the first use of shotgun proteomics to detect the protein expression profile of buccal swabs and cloth samples from two 500-year-old Andean mummies. The profile of one of the mummies is consistent with immune system response to severe pulmonary bacterial infection at the time of death. Presence of a probably pathogenic Mycobacterium sp. in one buccal swab was confirmed by DNA amplification, sequencing, and phylogenetic analyses. Our study provides positive evidence of active pathogenic infection in an ancient sample for the first time. The protocol introduced here is less susceptible to contamination than DNA-based or immunoassay-based studies. In scarce forensic samples, shotgun proteomics narrows the range of pathogens to detect using DNA assays, reducing cost. This analytical technique can be broadly applied for detecting infection in ancient samples to answer questions on the historical ecology of specific pathogens, as well as in medico-legal cases when active pathogenic infection is suspected.
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