ArticlePloS one2012
N-carbamylglutamate enhances pregnancy outcome in rats through activation of the PI3K/PKB/mTOR signaling pathway.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 2 syntheses or guidelines pooled it, 63 citations in OpenAlex.
- Prenatal Amino Acid Supplementation to Improve Fetal Growth: A Systematic Review and Meta-Analysis.Nutrients · 2020Pooled it
- Pooled it
- N-Carbamylglutamate Improves Production Performance and Muscle Growth by Regulating Protein Digestive Function and Muscle Protein Synthesis in Broiler Chickens.Animals : an open access journal from MDPI · 2026Article
- Activation of mitochondrial CPS1 promotes dormant ovarian follicle activation via arginine elevation and the mTORC1 pathway.Frontiers in cell and developmental biology · 2026Article
- N-carbamylglutamate (NCG) supplementation in low-protein diets improving egg quality by regulating protein metabolism pathway of oviduct in laying hens.Frontiers in cell and developmental biology · 2026Article
- Metabolomic profiling reveals amino acid dysregulation in congenital heart disease: Arginine-induced embryonic malformations and pathogenic insights.Biomedical reports · 2025Article
- Supplementation of Arginine or N-Carbamylglutamate Affects Jejunum Development, Global Arginine Bioavailability Ratio, and Stress-Related Indices in Young Rex Rabbits.Animals : an open access journal from MDPI · 2025Article
- Protective effect of leukemia inhibitory factor on the retinal injury induced by acute ocular hypertension in rats.Experimental and therapeutic medicine · 2023Article
- Article
- A review of the amino acid metabolism in placental function response to fetal loss and low birth weight in pigs.Journal of animal science and biotechnology · 2022Review
- DietaryAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
- Dietary rumen-protected L-arginine orAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Article
- Programming of Embryonic Development.International journal of molecular sciences · 2021Review
- Placental Impact of Dietary Supplements: More Than Micronutrients.Clinical therapeutics · 2021Review
- CBS and MAT2A improve methionine-mediated DNA synthesis through SAMTOR/mTORC1/S6K1/CAD pathway during embryo implantation.Cell proliferation · 2021Article
- Review
- Role of Dietary Amino Acids and Nutrient Sensing System in Pregnancy Associated Disorders.Frontiers in pharmacology · 2020Review
- Effect of dietary N-carbamylglutamate on development of ovarian follicles via enhanced angiogenesis in the chicken.Poultry science · 2020Article
- GNG7 silencing promotes the proliferation and differentiation of placental cytotrophoblasts in preeclampsia rats through activation of the mTOR signaling pathway.International journal of molecular medicine · 2019Article
- Advances in low-protein diets for swine.Journal of animal science and biotechnology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Administration of N-carbamylglutamate (NCG), an analogue of endogenous N-acetyl-glutamate (an activator of arginine synthesis) has been shown to enhance neonatal growth by increasing circulating arginine levels. However, the effect of NCG on pregnancy remains unknown. This study examined the effects of NCG on pregnancy outcome and evaluated potential mechanisms involved. Reproductive performance, embryo implantation, and concentration of amino acids in serum and uterine flushing, were determined in rats fed control or NCG supplemented diets. Ishikawa cells and JAR cells were used to examine the mechanism by which NCG affects embryo implantation. Dietary NCG supplementation increased serum levels of arginine, onithine, and proline, as well as uterine levels of arginine, glutamine, glutamate, and proline. Additionally, it stimulated LIF expression, and enhanced the activation of signal transduction and activator of transcription 3 (Stat3), protein kinase B (PKB), and 70-kDa ribosomal protein S6 kinase (S6K1) during the periimplantation period, resulting in an increase in litter size but not birth weight. In uterine Ishikawa cells, LIF expression was also enhanced by treatment with arginine and its metabolites. In trophoblast JAR cells, treatment with arginine and its metabolites enhanced Stat3, PKB, and S6K1 activation and facilitated cellular adhesion activity. These effects were abolished by pretreatment with inhibitors of phosphatidylinositol 3-kinase (wortmannin) and mammalian target of rapamycin (rapamycin). The results demonstrate that NCG supplementation enhances pregnancy outcome and have important implications for the pregnancy outcome of mammalian species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.