ArticlePloS one2012
An effort to use human-based exome capture methods to analyze chimpanzee and macaque exomes.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Cross-Species Exome Sequencing Reveals Recurrent Genomic Alterations in California Sea Lion (Genes · 2026Article
- Divergent evolution of hepatocellular carcinoma genomes in chimpanzees and humans.Evolution, medicine, and public health · 2026Article
- Genome-scale evolution in local populations of wild chimpanzees.Scientific reports · 2025Article
- Exome sequencing of hepatocellular carcinoma in lemurs identifies potential cancer drivers: A pilot study.Evolution, medicine, and public health · 2022Article
- The Use and Limitations of Exome Capture to Detect Novel Variation in the Hexaploid Wheat Genome.Frontiers in plant science · 2022Article
- Efficiency of whole-exome sequencing in old world and new world primates using human capture reagents.Journal of medical primatology · 2021Article
- Urine as a high-quality source of host genomic DNA from wild populations.Molecular ecology resources · 2021Article
- The Distribution and Origins ofFrontiers in plant science · 2021Article
- Genomic targets for high-resolution inference of kinship, ancestry and disease susceptibility in orang-utans (genus: Pongo).BMC genomics · 2020Article
- A review of research in primate sanctuaries.Biology letters · 2020Review
- Comparative population genomics reveals genetic divergence and selection in lotus, Nelumbo nucifera.BMC genomics · 2020Article
- Phylogenomic Insights into Mouse Evolution Using a Pseudoreference Approach.Genome biology and evolution · 2017Article
- Targeted Capture of Complete Coding Regions across Divergent Species.Genome biology and evolution · 2017Article
- Species specific exome probes reveal new insights in positively selected genes in nonhuman primates.Scientific reports · 2016Article
- Design and application of a target capture sequencing of exons and conserved non-coding sequences for the rat.BMC genomics · 2016Article
- Targeted capture in evolutionary and ecological genomics.Molecular ecology · 2016Review
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- Unlocking the vault: next-generation museum population genomics.Molecular ecology · 2013Article
- The value of extended pedigrees for next-generation analysis of complex disease in the rhesus macaque.ILAR journal · 2013Review
Corrections and comments
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Authors and funding
35 authors at 12 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Non-human primates have emerged as an important resource for the study of human disease and evolution. The characterization of genomic variation between and within non-human primate species could advance the development of genetically defined non-human primate disease models. However, non-human primate specific reagents that would expedite such research, such as exon-capture tools, are lacking. We evaluated the efficiency of using a human exome capture design for the selective enrichment of exonic regions of non-human primates. We compared the exon sequence recovery in nine chimpanzees, two crab-eating macaques and eight Japanese macaques. Over 91% of the target regions were captured in the non-human primate samples, although the specificity of the capture decreased as evolutionary divergence from humans increased. Both intra-specific and inter-specific DNA variants were identified; Sanger-based resequencing validated 85.4% of 41 randomly selected SNPs. Among the short indels identified, a majority (54.6%-77.3%) of the variants resulted in a change of 3 base pairs, consistent with expectations for a selection against frame shift mutations. Taken together, these findings indicate that use of a human design exon-capture array can provide efficient enrichment of non-human primate gene regions. Accordingly, use of the human exon-capture methods provides an attractive, cost-effective approach for the comparative analysis of non-human primate genomes, including gene-based DNA variant discovery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.